Strenge Jan Tinson, Smeets Ralf, Geffken Maria, Beikler Thomas, Stuermer Ewa Klara
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Dent J (Basel). 2025 Jul 16;13(7):324. doi: 10.3390/dj13070324.
: The formation of oral biofilms in periodontal pockets and around dental implants with induction of periodontitis or peri-implantitis is an increasing problem in dental health. The intelligent design of a biofilm makes the bacteria embedded in the biofilm matrix highly tolerant to antiseptic therapy, often resulting in tooth or implant loss. The question therefore arises as to which mouthwashes have eradication potential against oral biofilm. : A human oral biofilm model was developed based on donated blood plasma combined with buffy coats, inoculated with oral pathogenic bacterial species found in periodontal disease (, , , and ). Over a span of 7 days, we tested different mouth rinsing and antiseptic solutions (Chlorhexidine, Listerine, NaOCl, Octenisept, and Octenident) covering the matured biofilm with 24 h renewal. Phosphate-buffered saline (PBS) was used as a control. Bacterial growth patterns were detected via quantitative polymerase chain reaction (qPCR) after 2, 4, and 7 days of treatment. : While all groups showed initial bacterial reduction, the control group demonstrated strong regrowth from day 2 to 4. Listerine showed a near-significant trend toward bacterial suppression. Additionally, strain-specific efficacy was observed, with Octenisept being most effective against , Octenident and NaOCl showing superior suppression of , and Listerine outperforming other solutions in reducing . Donor-specific, individual variability further influenced treatment outcomes, with distinct trends in bacterial suppression and regrowth observed across donors. : These findings underscore the complexity of biofilm-associated infections and highlight the importance of targeted therapeutic approaches for managing bacterial biofilms. In this experiment, the donor-specific outcomes of the antimicrobial effects of the solutions may indicate that genetic predisposition/tolerance to oral infections appears to play a critical role in the control of oral biofilms.
在牙周袋以及伴有牙周炎或种植体周围炎的牙种植体周围形成口腔生物膜，是口腔健康领域日益严重的问题。生物膜的智能设计使得嵌入生物膜基质中的细菌对抗菌治疗具有高度耐受性，常常导致牙齿或种植体脱落。因此，问题就出现了，即哪种漱口水具有根除口腔生物膜的潜力。
基于捐献的血浆与血沉棕黄层相结合开发了一种人类口腔生物膜模型，并接种了牙周疾病中发现的口腔致病细菌物种（、、和）。在7天的时间里，我们测试了不同的漱口液和抗菌溶液（洗必泰、李斯德林、次氯酸钠、奥替尼啶和奥克泰士），每24小时更换一次，覆盖成熟生物膜。使用磷酸盐缓冲盐水（PBS）作为对照。在治疗2、4和7天后，通过定量聚合酶链反应（qPCR）检测细菌生长模式。
虽然所有组最初都显示出细菌数量减少，但对照组在第2天到第4天显示出强烈的再生长。李斯德林显示出接近显著的细菌抑制趋势。此外，观察到了菌株特异性疗效，奥替尼啶对最有效，奥克泰士和次氯酸钠对显示出更好的抑制效果，而李斯德林在减少方面优于其他溶液。供体特异性的个体差异进一步影响了治疗结果，不同供体在细菌抑制和再生长方面观察到了明显的趋势。
这些发现强调了生物膜相关感染的复杂性，并突出了针对细菌生物膜管理的靶向治疗方法的重要性。在本实验中，溶液抗菌作用的供体特异性结果可能表明，对口腔感染的遗传易感性/耐受性似乎在口腔生物膜的控制中起着关键作用。
