Robertson R T, Höhmann C F, Bruce J L, Coyle J T
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine 92717.
Brain Res. 1988 Apr 1;467(2):298-302. doi: 10.1016/0165-3806(88)90034-x.
Infant rats were subjected to unilateral or bilateral enucleation or sham lesions on the day of birth. On postnatal day 13 or 14, tissue blocks from cortex were assayed for acetylcholinesterase (AChE) and choline acetyltransferase (ChAT). Bilateral enucleation resulted in a decrease in specific activity of AChE in occipital cortex, as compared with control animals. Unilateral enucleation resulted in a decrease of AChE activity in the hemisphere contralateral to the enucleated orbit. However, neither type of enucleation resulted in significant changes of ChAT specific activity. These data suggest that reductions in AChE activity resulting from neonatal enucleations are not due to a direct involvement of cholinergic afferents to visual cortex.
出生当天，将新生大鼠进行单侧或双侧眼球摘除术或假损伤处理。在出生后第13或14天，对取自皮质的组织块进行乙酰胆碱酯酶（AChE）和胆碱乙酰转移酶（ChAT）检测。与对照动物相比，双侧眼球摘除导致枕叶皮质中AChE的比活性降低。单侧眼球摘除导致摘除侧眼眶对侧半球的AChE活性降低。然而，两种类型的眼球摘除均未导致ChAT比活性发生显著变化。这些数据表明，新生儿眼球摘除所致的AChE活性降低并非由于胆碱能传入纤维直接参与视觉皮质所致。
