Mivechi N F
Department of Radiation Research, City of Hope National Medical Center, Duarte, California 91010.
Cancer Res. 1988 Jul 1;48(13):3630-3.
Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43 degrees C, 44 degrees C, and 45 degrees C were 23, 12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44 degrees C/20 min, 45 degrees C/10 min, 41 degrees C/2 h, and 42 degrees C/1 h and 4 h. The cells were then challenged with 44 degrees C or 45 degrees C after incubation for 2, 4, 6, 24, and 48 h at 37 degrees C. In all cases, except for 41 degrees C/2 h, thermotolerance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41 degrees C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44 degrees C/20 min or 45 degrees C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
分别在人粒细胞-巨噬细胞祖细胞和有核骨髓细胞中研究了热敏感性、热耐受诱导和衰减的动力学以及热休克蛋白合成情况。人粒细胞-巨噬细胞集落形成细胞在43℃、44℃和45℃时热存活曲线的D0分别为23分钟、12分钟和5分钟。热耐受诱导的动力学通过44℃/20分钟、45℃/10分钟、41℃/2小时、42℃/1小时和42℃/4小时的触发剂量来测定。然后在37℃孵育2小时、4小时、6小时、24小时和48小时后，用44℃或45℃对细胞进行刺激。在所有情况下，除了41℃/2小时，热耐受在2至6小时达到最大值，在24小时开始衰减，并在48小时完全衰减。在41℃/2小时的触发剂量下，热耐受在24小时完全衰减。在44℃/20分钟或45℃/10分钟的触发剂量后测定热休克蛋白合成。在初始加热后至少8小时内，总核骨髓细胞中分子量为70000和87000的热休克蛋白的合成明显。
