Furness J B, Keast J R, Pompolo S, Bornstein J C, Costa M, Emson P C, Lawson D E
Department of Anatomy and Histology, Flinders University, South Australia.
Cell Tissue Res. 1988 Apr;252(1):79-87. doi: 10.1007/BF00213828.
Immunoreactivity for vitamin D-dependent calcium-binding protein (CaBP) has been localized in nerve cell bodies and nerve fibres in the gastrointestinal tracts of guinea-pig, rat and man. CaBP immunoreactivity was found in a high proportion of nerve cell bodies of the myenteric plexus, particularly in the small intestine. It was also found in submucous neurons of the small and large intestines. Immunoreactive nerve fibres were numerous in the myenteric ganglia, and were also common in the submucous ganglia and in the intestinal mucosa. Immunoreactive fibres were rare in the circular and longitudinal muscle coats. In the myenteric ganglia of the guinea-pig small intestine the immunoreactivity is restricted to one class of nerve cell bodies, type-II neurons of Dogiel, which display calcium action potentials in their cell bodies. These neurons were also immunoreactive with antibodies to spot 35 protein, a calcium-binding protein from the cerebellum. From the distribution of their terminals and the electrophysiological properties of these neurons it is suggested they might be sensory neurons, or perhaps interneurons. The discovery of CaBP in restricted sub-groups of enteric neurons may provide an important key for the analysis of their functions.
维生素D依赖性钙结合蛋白（CaBP）的免疫反应性已定位在豚鼠、大鼠和人类胃肠道的神经细胞体和神经纤维中。在肌间神经丛的高比例神经细胞体中发现了CaBP免疫反应性，尤其是在小肠中。在小肠和大肠的黏膜下神经元中也发现了这种反应性。免疫反应性神经纤维在肌间神经节中大量存在，在黏膜下神经节和肠黏膜中也很常见。在环形肌层和纵行肌层中免疫反应性纤维很少。在豚鼠小肠的肌间神经节中，免疫反应性仅限于一类神经细胞体，即多吉尔II型神经元，其细胞体显示钙动作电位。这些神经元也与针对斑点35蛋白的抗体发生免疫反应，斑点35蛋白是一种来自小脑的钙结合蛋白。从它们的终末分布和这些神经元的电生理特性来看，提示它们可能是感觉神经元，或者也许是中间神经元。在肠道神经元的特定亚群中发现CaBP可能为分析它们的功能提供重要线索。
