McConalogue K, Low A M, Williamson S, Bornstein J C, Furness J B
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Australia.
Cell Tissue Res. 1994 May;276(2):359-65. doi: 10.1007/BF00306121.
The distribution of nerve cells and fibres with immunoreactivity for the calcium-binding protein, calretinin, was studied in the distal colon of the guinea-pig. The projections of the neurons were determined by examining the consequences of lesioning the myenteric plexus. Calretinin-immunoreactive neurons comprised 17% of myenteric nerve cells and 6% of submucous nerve cells. Numerous calretinin-immunoreactive nerve fibres were located in the longitudinal and circular muscle, and within the ganglia of the myenteric and submucous plexuses. Occasional fibres were found in the muscularis mucosae, but they were very rare in the lamina propria of the mucosa. Lesion studies revealed that myenteric neurons innervated the underlying circular muscle and provided both ascending and descending processes that gave rise to varicose branches in myenteric ganglia. Calretinin-immunoreactive fibres also projected to the tertiary component of the myenteric plexus, and are therefore likely to be motor neurons to the longitudinal muscle. Varicose fibres that supplied the submucous ganglia appear to arise from submucous nerve cells. Arterioles of the submucous plexus were sparsely innervated by calretinin-immunoreactive fibres. The submucous plexus was the principal source of immunoreactive nerve fibres in the muscularis mucosae. This work shows that calretinin-IR reveals different neuronal populations in the large intestine to those previously reported in the small intestine.
研究了豚鼠远端结肠中具有钙结合蛋白钙视网膜蛋白免疫反应性的神经细胞和纤维的分布。通过检查损伤肌间神经丛的后果来确定神经元的投射。钙视网膜蛋白免疫反应性神经元占肌间神经细胞的17%，占黏膜下神经细胞的6%。许多钙视网膜蛋白免疫反应性神经纤维位于纵行肌和环行肌以及肌间和黏膜下神经丛的神经节内。在黏膜肌层中偶尔发现纤维，但在黏膜固有层中非常罕见。损伤研究表明，肌间神经元支配其下方的环行肌，并提供升支和降支，这些分支在肌间神经节中形成曲张分支。钙视网膜蛋白免疫反应性纤维也投射到肌间神经丛的第三成分，因此可能是纵行肌的运动神经元。供应黏膜下神经节的曲张纤维似乎起源于黏膜下神经细胞。黏膜下神经丛的小动脉稀疏地接受钙视网膜蛋白免疫反应性纤维的支配。黏膜下神经丛是黏膜肌层中免疫反应性神经纤维的主要来源。这项研究表明，钙视网膜蛋白免疫反应性揭示了大肠中与先前在小肠中报道的不同的神经元群体。
