Laboratory of Analysis of Natural Compounds, Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308 Gdańsk, Poland.
Department of Plant Protection, Institute of Agriculture and Horticulture, Siedlce University of Natural Sciences and Humanities, B. Prusa 14, 08-110 Siedlce, Poland.
Mycologia. 2021 May-Jun;113(3):525-535. doi: 10.1080/00275514.2021.1877520. Epub 2021 Mar 30.
is a pest of the honey bee ( L.) and causes significant losses to the beekeeping industry; therefore, experiments are needed to decode the effects of entomopathogenic fungi on insect physiology. The gas chromatography-mass spectrometry (GC-MS) method was successful for the determination of the organic compounds of larvae, noninfected and infected by three fungal species: , and . A total of 46 compounds were identified in , including fatty acids, other acids, fatty acid methyl esters, monoacylglycerols, amino acids, sterols, and several other organic compounds. The lipids of larvae after , and exposure contained 40, 35, and 33 organic compounds, respectively. The following organic compounds, present in the noninfected larvae, were absent from the infected larvae: fatty acids C22:0 and C24:0, glutaric acid, urocanic acid, hydroxycinnamic acid, dihydroxycinnamic acid, 10-oxodecanoic acid, glycine, aspartic acid, glutamic acid, lysine, tyrosine, tryptophan, 2-aminobutyric acid, and tyramine. These compounds can be used by fungi as an exogenous source of carbon. The following organic compounds, present in the infected larvae, were absent from the noninfected larvae: fatty acids C10:0, C11:0, C13:0, and C20:0, suberic acid, phenylacetic acid, fatty acid methyl ester (FAME) C16:0, FAME C18:2, FAME C18:1, glycerol 2-monopalmitate, norvaline, proline, sitosterol, and 2-dekanal. These compounds can be synthesized as an insect response to fungal infection.
是蜜蜂（L.）的害虫，给养蜂业造成重大损失；因此，需要进行实验来解码昆虫病原真菌对昆虫生理学的影响。气相色谱-质谱（GC-MS）法成功地测定了幼虫、未感染和感染三种真菌（，和）的有机化合物。在幼虫中鉴定出 46 种化合物，包括脂肪酸、其他酸、脂肪酸甲酯、单酰基甘油、氨基酸、固醇和几种其他有机化合物。幼虫暴露在、和后，其脂质分别含有 40、35 和 33 种有机化合物。以下有机化合物存在于未感染的幼虫中，但不存在于感染的幼虫中：脂肪酸 C22:0 和 C24:0、戊二酸、尿刊酸、羟基肉桂酸、二羟基肉桂酸、10-氧代癸酸、甘氨酸、天冬氨酸、谷氨酸、赖氨酸、酪氨酸、色氨酸、2-氨基丁酸和酪胺。这些化合物可被真菌用作外源碳源。以下有机化合物存在于感染的幼虫中，但不存在于未感染的幼虫中：脂肪酸 C10:0、C11:0、C13:0 和 C20:0、琥珀酸、苯乙酸、脂肪酸甲酯（FAME）C16:0、FAME C18:2、FAME C18:1、甘油 2-单棕榈酸酯、正缬氨酸、脯氨酸、谷甾醇和 2-癸醛。这些化合物可能是昆虫对真菌感染的反应而合成的。
