Koyama I, Arai Y, Miura M, Matsuzaki H, Sakagishi Y, Komoda T
Department of Biochemistry, Saitama Medical School, Tokyo, Japan.
Clin Chim Acta. 1988 Apr 15;173(2):139-46. doi: 10.1016/0009-8981(88)90251-3.
Characteristics of human renal cortex and medulla alkaline phosphatase (ALP) were compared. Enzymatic and hydrophobic properties of both ALPs were almost similar. However, the results of concanavalin A affinity chromatography and wheat germ agglutinin affinity electrophoresis, exhibited that sugar chain structure(s) might be different between the cortex and medulla ALPs. In addition, the molecular mass and substrate specificity differed from each other, and these results of cortex and medulla ALPs were well accordant with those of human duodenal and ileal ALPs, respectively, as described previously (Clin Chim Acta 1987;163:279-287).
对人肾皮质和髓质碱性磷酸酶（ALP）的特性进行了比较。两种ALP的酶学和疏水特性几乎相似。然而，伴刀豆球蛋白A亲和层析和麦胚凝集素亲和电泳的结果表明，皮质和髓质ALP的糖链结构可能不同。此外，两者的分子量和底物特异性也互不相同，而且皮质和髓质ALP的这些结果分别与先前所述的人十二指肠和回肠ALP的结果非常一致（《临床化学学报》1987年；163:279 - 287）。
