在鸽子过敏原阳性哮喘患者中 MUC1 蛋白的表达谱

Expression profile of MUC1 protein in Pigeon allergens positive asthmatic.

机构信息

Department of Pulmonary Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.

School of Biosciences, IMS Ghaziabad, NH9 Ghaziabad 201015, Uttar Pradesh, India.

出版信息

Immunobiology. 2021 May;226(3):152086. doi: 10.1016/j.imbio.2021.152086. Epub 2021 Mar 20.

DOI:10.1016/j.imbio.2021.152086

PMID:33784545

Abstract

BACKGROUND

Certain urban areas could contain many pigeon's allergens, which may play an imperative role in the exacerbation of asthma in pigeon allergen sensitive asthma patients. The circulating form of MUC1 in human serum has been considered as a biomarker for some allergic diseases. The study aimed to investigate the role of MUC1 in pigeon allergens positive asthma patients.

METHODS

We were enrolled 200 asthma patients including 81 males and 119 females. After positive pigeon exposure history, 108 patients underwent SPT testing against pigeon allergens (dropping and feather). A total of 17 patients, who had exposure history with SPT positive were undergone detail clinical examination. Serum MUC1expression analysis was done by western blotting method.

RESULTS

Out of 200 asthmatic patients, 108 (54%) patients had a history of exposure to pigeons. Skin prick test against pigeon (feather & dropping) allergens was positive in 17 (15.7%) patients among exposure asthmatics. The mean age of the study population was 28.8 ± 10.4 years with 9 males and 8 females. Baseline airway obstruction was seen in 58.8% cases. Out of 17 pigeons expose and sensitive asthmatic the MUC1 expression was up-regulated in 15 (88.2%) and down-regulated in 2 (11.8%). The mean value MUC1 fold change of 15 patients with up-regulation was 4.63 ± 3.00 fold.

CONCLUSION

MUC1 expression was up-regulated in 88.2% of patients, who were exposed and sensitive to pigeon allergen (dropping and feather). MUC1 may consider as a biomarker in pigeon sensitive asthma patients.

摘要

背景

某些城市地区可能含有许多鸽子过敏原，这些过敏原可能在鸽子过敏原敏感哮喘患者的哮喘恶化中起重要作用。人血清中的 MUC1 循环形式已被认为是某些过敏性疾病的生物标志物。本研究旨在探讨 MUC1 在鸽子过敏原阳性哮喘患者中的作用。

方法

我们纳入了 200 例哮喘患者，包括 81 名男性和 119 名女性。在有阳性鸽子暴露史后，108 例患者接受了针对鸽子过敏原（羽毛和粪便）的 SPT 检测。共有 17 例有暴露史且 SPT 阳性的患者接受了详细的临床检查。采用 Western blot 法检测血清 MUC1 表达。

结果

在 200 例哮喘患者中，有 108 例（54%）有接触鸽子的病史。在有暴露史的哮喘患者中，有 17 例（15.7%）对鸽子（羽毛和粪便）过敏原的皮肤点刺试验阳性。研究人群的平均年龄为 28.8±10.4 岁，其中 9 名为男性，8 名为女性。58.8%的患者存在基线气道阻塞。在 17 例暴露于鸽子且敏感的哮喘患者中，MUC1 的表达上调 15 例（88.2%），下调 2 例（11.8%）。MUC1 表达上调的 15 例患者的平均 MUC1 倍数变化为 4.63±3.00 倍。