Feurle G E, Tischbirek K, Baća I
Medizinische Poliklinik, University of Heidelberg, Federal Republic of Germany.
Dig Dis Sci. 1988 Jul;33(7):795-800. doi: 10.1007/BF01550965.
Since little is known about the pathophysiology of pyloric stenosis, we created a partial gastric outlet obstruction in 13 Wistar rats by placing a nonabsorbable ligature of defined size around the pylorus. Sham operations were performed in 10 rats. The animals from both groups were killed after four months. G-cell count and gastrin content were determined in 10 parallel strips, which were cut by razor blades mounted on a handle. Gastric size and weight as well as thickness of mucosal and muscular layers and serum gastrin concentration were also determined. Body weight of the animals with pyloric stenosis was lower and gastric weight higher than that of the controls. Furthermore, we found an enlarged G-cell area and G-cell hyperplasia, an increased surface area and thickness of the mucosal and muscular layers of the stomach, and in the majority of rats, elevated serum gastrin levels. Total G-cell count was 583,720 +/- 90,561 in the rats with pyloric stenosis and 385,775 +/- 15,820 (mean +/- SEM) in the control rats (P less than 0.04). We conclude that partial gastric outlet obstruction in rats leads to G-cell hyperplasia and that this experiment may serve as a model for pyloric stenosis in man.
由于对幽门狭窄的病理生理学了解甚少，我们通过在13只Wistar大鼠的幽门周围放置尺寸确定的不可吸收结扎线，制造了部分胃出口梗阻。对10只大鼠进行了假手术。两组动物在四个月后处死。在由安装在手柄上的剃须刀片切割的10条平行条带中测定G细胞计数和胃泌素含量。还测定了胃的大小、重量以及粘膜和肌层的厚度和血清胃泌素浓度。幽门狭窄动物的体重低于对照组，胃重量高于对照组。此外，我们发现G细胞面积增大和G细胞增生，胃粘膜和肌层的表面积和厚度增加，并且在大多数大鼠中，血清胃泌素水平升高。幽门狭窄大鼠的G细胞总数为583,720±90,561，对照大鼠为385,775±15,820（平均值±标准误）（P<0.04）。我们得出结论，大鼠的部分胃出口梗阻会导致G细胞增生，并且该实验可作为人类幽门狭窄的模型。
