Romanelli L, Ohman B, Adem A, Nordberg A
Department of Pharmacology, University of Uppsala, Sweden.
Eur J Pharmacol. 1988 Mar 29;148(2):289-91. doi: 10.1016/0014-2999(88)90577-8.
A significant increase in the number of cortical high-affinity (-)-[3H]nicotine binding sites was measured in rats treated with nicotine (0.45 mg/kg) twice daily for 18 days. Competition experiments with (-)-[3H]nicotine and various concentrations of unlabelled (-)-nicotine revealed that the proportion of high-affinity nicotine binding sites was significantly increased in the nicotine-treated group while the proportion of low-affinity nicotinic binding sites was similarly significantly reduced compared to the controls. In addition there was a significant decrease in the affinity of both subtypes of nicotinic binding sites.
每天两次给大鼠注射尼古丁（0.45毫克/千克），持续18天，结果发现其大脑皮质中高亲和力的（-）-[3H]尼古丁结合位点数量显著增加。用（-）-[3H]尼古丁和不同浓度的未标记（-）-尼古丁进行竞争实验表明，与对照组相比，尼古丁处理组中高亲和力尼古丁结合位点的比例显著增加，而低亲和力烟碱结合位点的比例同样显著降低。此外，两种亚型烟碱结合位点的亲和力均显著下降。
