琼脂平板培养：一种用于检测蛔虫和钩虫寄生虫的替代灵敏常规实验室检测方法。
Agar Plate Culture: An Alternative Sensitive Routine Laboratory Detection Method for and Hookworm Parasites.
作者信息
Hailu Tadesse, Nibret Endalkachew, Amor Arancha, Munshea Abaineh, Anegagrie Melaku
机构信息
Department of Medical Laboratory Science, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia.
Biology Department, Science College, Bahir Dar University, Bahir Dar, Ethiopia.
出版信息
Iran J Parasitol. 2021 Jan-Mar;16(1):72-80. doi: 10.18502/ijpa.v16i1.5514.
BACKGROUND
Human infection with and hookworm parasites is usually under reported due to less sensitive diagnostic methods. Agar plate culture (APC) is the most sensitive technique for parasites having larval stage. However, using APC in routine diagnosis is uncommon. This study aimed to determine the detection rate and sensitivity of APC in comparison with formal ether concentration technique (FECT) and spontaneous tube sedimentation techniques (STSTs) for and hookworm larvae.
METHODS
Stool samples collected from 844 schoolchildren in Amhara Regional State, northwestern Ethiopia in 2019, transported to nearby health institutions and processed by APC, FECT and STSTs. The prevalence of and hookworm was computed by descriptive statistics and Chi-square. The diagnostic agreement among the three techniques was evaluated using Kappa value.
RESULTS
The overall prevalence of and hookworm infections by combining the three methods was 13.2% (111/844) and 33.8% (277/844), respectively. Using APC alone, the prevalence of and hookworm were found to be 10.9% (92/844) and 24.5% (207/844), respectively. Agar plate culture was 5.4 and 2.7 times respectively more sensitive than FECT and STST, with slight and fair agreement in the detection of Hookworm diagnostic agreement was moderate between APC and FECT, and APC and STST. The Kappa value between STST and FECT diagnostic methods was substantial.
CONCLUSION
APC has a better detection rate of and hookworm larvae. Therefore, APC can be used as an alternative routine diagnostic method to and hookworm co-endemic countries.
背景
由于诊断方法不够灵敏，人体感染蛔虫和钩虫寄生虫的情况通常报告不足。琼脂平板培养（APC）是检测具有幼虫阶段寄生虫的最灵敏技术。然而，在常规诊断中使用APC并不常见。本研究旨在确定与甲醛乙醚浓缩技术（FECT）和自然沉淀法（STST）相比，APC对蛔虫和钩虫幼虫的检出率及灵敏度。
方法
2019年从埃塞俄比亚西北部阿姆哈拉州的844名学童中采集粪便样本，运至附近医疗机构，采用APC、FECT和STST进行处理。通过描述性统计和卡方检验计算蛔虫和钩虫的感染率。使用Kappa值评估三种技术之间的诊断一致性。
结果
三种方法联合检测时，蛔虫和钩虫感染的总体患病率分别为13.2%（111/844）和33.8%（277/844）。单独使用APC时，蛔虫和钩虫的患病率分别为10.9%（92/844）和24.5%（207/844）。琼脂平板培养对蛔虫和钩虫的检测灵敏度分别比FECT和STST高5.4倍和2.7倍，在检测蛔虫方面一致性一般，在检测钩虫方面一致性中等。APC与FECT、APC与STST之间的诊断一致性中等。STST和FECT诊断方法之间的Kappa值较高。
结论
APC对蛔虫和钩虫幼虫的检出率更高。因此，在蛔虫和钩虫共同流行的国家，APC可作为一种替代的常规诊断方法。
Zotero 插件