Zoology Department, Faculty of Science, King Saud University, P.O. Box 2454, Riyadh, 11451, Kingdom of Saudi Arabia.
Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
BMC Complement Altern Med. 2018 Apr 27;18(1):135. doi: 10.1186/s12906-018-2191-z.
Schistosomiasis is an acute and chronic zoonotic parasitic disease caused by trematode worms. The host inflammatory response to schistosome eggs leads to perioval granulomata formation, mainly in the liver and intestine. This study investigated the potential antischistosomal and anti-inflammatory activity of both garlic extract and allicin on liver fibrotic markers in BALB/c mice with schistosomiasis (S. mansoni infection) compared with that of the commonly used drug, praziquantel (PZQ).
In this study, 140 female BALB/c mice (7-weeks old) were divided into seven groups with 20 mice each. Six groups were infected with S. mansoni cercariae and treated with garlic, allicin, or PZQ. The seventh group was the negative control. Twenty-four hours after the final treatment, the mice were euthanised and perfused for worm recovery. The liver and intestines were harvested for parasitological and histological assessment and to analyse the proinflammatory cytokine mRNA expression.
Prophylactic administration of garlic and allicin to the infected mice significantly reduced the worm burden. Serum concentrations of liver fibrosis markers and proinflammatory cytokines were also reduced. PZQ was the most efficacious for reduction in the number of worms. These results are similar to those normally obtained using PZQ.
Crushed garlic homogenate and allicin are potential complementary treatments that may be used with PZQ.
血吸虫病是一种由吸虫引起的急性和慢性人畜共患寄生虫病。宿主对血吸虫卵的炎症反应导致周围肉芽肿形成，主要在肝脏和肠道。本研究调查了大蒜提取物和大蒜素对感染曼氏血吸虫（S. mansoni）的 BALB/c 小鼠肝纤维化标志物的潜在抗血吸虫和抗炎活性，并与常用药物吡喹酮（PZQ）进行了比较。
本研究将 140 只 7 周龄雌性 BALB/c 小鼠分为 7 组，每组 20 只。6 组感染曼氏血吸虫尾蚴，并给予大蒜、大蒜素或 PZQ 治疗。第 7 组为阴性对照。最后一次治疗后 24 小时，处死小鼠并进行灌流以回收蠕虫。采集肝脏和肠道进行寄生虫学和组织学评估，并分析促炎细胞因子 mRNA 表达。
预防性给予感染小鼠大蒜和大蒜素可显著降低虫体负荷。血清肝纤维化标志物和促炎细胞因子浓度也降低。PZQ 对减少蠕虫数量最有效。这些结果与通常使用 PZQ 获得的结果相似。
粉碎的大蒜匀浆和大蒜素是可能与 PZQ 联合使用的潜在补充治疗方法。
