Golovkin V D, Gukasov V M, Gatsura V V
Farmakol Toksikol. 1988 Mar-Apr;51(2):56-9.
Effects of obsidan and isoptine on Fe2+-induced free radical lipid peroxidation (FRLP) were studied according to the data of chemoluminiscence (CL) and malon dialdehyde (MDA) content during experiments on the intact myocardium and at two-thour ischemia of the myocardium as well in the suspension of egg yolk lipoproteids. It was found that in the intact myocardium obsidan (10% LD50) suppressed FRLP according to the CL data, simultaneously increasing MDA level, and in the ischemic myocardium significantly suppressed FRLP by a number of parameters. Isoptine in the intact myocardium exerted no effect on CL with a simultaneous increase of MDA level, and in the ischemic myocardium suppressed FRLP by most parameters. In the suspension of egg yolk lipoproteids obsidan and isoptine changed MDA concentration in a two-phase manner. Isoptine also had a two-phase action on FRLP according to the CL data. A high antioxidative activity of obsidan in the suspension of egg yolk lipoproteids was noted as compared to that of isoptine. Based on the data of kinetics of Fe2+-induced CL in the suspension of egg yolk lipoproteids, obsidan is a true antioxidant by its mechanism of action and ispoptine is a structural antioxidant.
根据化学发光（CL）数据和丙二醛（MDA）含量，研究了黑曜石和维拉帕米对Fe2 +诱导的自由基脂质过氧化（FRLP）的影响，实验对象包括完整心肌、心肌缺血两小时的情况以及蛋黄脂蛋白悬浮液。结果发现，在完整心肌中，根据CL数据，黑曜石（10% LD50）抑制FRLP，同时MDA水平升高；在缺血心肌中，黑曜石通过多个参数显著抑制FRLP。维拉帕米在完整心肌中对CL无影响，但MDA水平同时升高，在缺血心肌中，维拉帕米通过大多数参数抑制FRLP。在蛋黄脂蛋白悬浮液中，黑曜石和维拉帕米以两相方式改变MDA浓度。根据CL数据，维拉帕米对FRLP也有两相作用。与维拉帕米相比，黑曜石在蛋黄脂蛋白悬浮液中具有较高的抗氧化活性。根据蛋黄脂蛋白悬浮液中Fe2 +诱导CL的动力学数据，黑曜石按其作用机制是一种真正的抗氧化剂，而维拉帕米是一种结构抗氧化剂。
