Demidkina T V, Myagkikh I V, Antson A A, Harutyunyan E H
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
FEBS Lett. 1988 May 23;232(2):381-2. doi: 10.1016/0014-5793(88)80774-9.
Crystals of the apoenzyme of tyrosine phenol-lyase (EC 4.1.99.2), a pyridoxal 5'-phosphate-dependent enzyme from Citrobacter intermedius, have been grown by vapor diffusion of an ammonium sulfate solution to a protein solution. The crystals belong to space group P2(1)2(1)2, with dimensions of a = 75.5 A, b = 138.4 A and c = 94.1 A and diffract up to 2.7 A resolution. The asymmetric unit contains one half of the enzyme tetrameric molecule. Two heavy-atom derivatives of the crystals have been obtained.
来自中间柠檬酸杆菌的一种依赖于磷酸吡哆醛的酪氨酸酚裂解酶（EC 4.1.99.2）的脱辅基酶晶体，通过硫酸铵溶液向蛋白质溶液的气相扩散法生长而成。这些晶体属于空间群P2(1)2(1)2，晶胞参数a = 75.5 Å，b = 138.4 Å，c = 94.1 Å，衍射分辨率高达2.7 Å。不对称单元包含酶四聚体分子的一半。已获得该晶体的两种重原子衍生物。
