Al-Abbas Nouf S, Shaer Nehad A
Biology Department, Jumum College University, Umm Alqura University, Saudi Arabia.
Chemistry Department, Umm Al-Qura University, Saudi Arabia.
Heliyon. 2021 Mar 16;7(3):e06255. doi: 10.1016/j.heliyon.2021.e06255. eCollection 2021 Mar.
Chemotherapy remains to be the method of choice used by clinicians to treat acute myeloid leukemia (AML) patients. However, the most common problem usually faced in the course of treatment is multidrug resistance (MDR). Nowadays, combination therapy involving natural products as adjuvant therapy to chemotherapy and radiotherapy has been used for many of health problems. Coumarin is a natural compound with known chemotherapeutic activity, as well as other pharmacological properties. We focused on the combination of coumarin and doxorubicin in overcoming of drug-resistance in acute myeloid leukemia.
Cell viability, Apoptotic and necrotic cell death with FACS, oxidative stress detection, and protein expression analysis were used in this study.
Coumarin as a single drug exerts a significant cell death on Human acute myeloid leukemia (HL60); however, it does not show the same effect on drug-resistant acute myeloid leukemia (HL60/ADR). Comparing the effects of doxorubicin and coumarin as single drugs versus a combination of coumarin and doxorubicin showed a significant apoptotic cell death.
In AML patients, the development of multiple drug resistance (MDR) is the biggest challenge in treating AML patients. Combination therapy with coumarin may be a good choice to overcome the drug resistance in AML patients.
化疗仍然是临床医生治疗急性髓系白血病（AML）患者所采用的首选方法。然而，在治疗过程中通常面临的最常见问题是多药耐药（MDR）。如今，涉及天然产物作为化疗和放疗辅助治疗的联合疗法已被用于许多健康问题。香豆素是一种具有已知化疗活性以及其他药理特性的天然化合物。我们专注于香豆素与阿霉素联合用于克服急性髓系白血病的耐药性。
本研究采用细胞活力、流式细胞术检测凋亡和坏死细胞死亡、氧化应激检测以及蛋白质表达分析。
香豆素作为单一药物对人急性髓系白血病（HL60）细胞具有显著的细胞杀伤作用；然而，它对耐药急性髓系白血病（HL60/ADR）细胞并未显示出相同的效果。比较阿霉素和香豆素单一药物与香豆素和阿霉素联合用药的效果，结果显示联合用药具有显著的凋亡细胞死亡作用。
在AML患者中，多药耐药（MDR）的产生是治疗AML患者面临的最大挑战。香豆素联合疗法可能是克服AML患者耐药性的一个良好选择。
