School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore.
STAR Protoc. 2021 Mar 20;2(2):100397. doi: 10.1016/j.xpro.2021.100397. eCollection 2021 Jun 18.
Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs that contribute to aging and to determine their underlying functions in each tissue. We further describe methods to compare the lncRNA expression patterns and dynamic changes among multiple tissues. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).
衰老相关长链非编码 RNA 的综合分析一直较为缺乏，因为之前的研究主要集中在衰老过程中的蛋白质编码基因上。在此，我们描述了一个在衰老过程中对小鼠长链非编码 RNA 进行全器官分析的方案。我们提供了逐步的说明，以确定导致衰老的长链非编码 RNA，并确定它们在每个组织中的潜在功能。我们进一步描述了比较多个组织中长链非编码 RNA 表达模式和动态变化的方法。如需了解本方案使用和执行的完整详细信息，请参考 Zhou 等人（2020 年）。
