Miller S C, Pavlath G K, Blakely B T, Blau H M
Department of Pharmacology, Stanford University School of Medicine, California 94305-5332.
Genes Dev. 1988 Mar;2(3):330-40. doi: 10.1101/gad.2.3.330.
Major changes in cytoarchitecture and gene expression were induced in short-term heterokaryons. When human hepatocytes were fused with mouse muscle cells, the hepatocyte Golgi apparatus changed from its usual polar location to a uniformly circumnuclear location typical of striated muscle. Human liver albumin ceased to be expressed, and expression of the human muscle cell-surface antigen 5.1H11 was induced without DNA replication or cell division. Coexpression of liver and muscle proteins was rarely observed. These novel findings provide insight into the regulation of gene expression and the targeting and localization of organelles with a central role in cell polarity, intracellular transport, and secretion.
短期异核体中诱导了细胞结构和基因表达的重大变化。当人类肝细胞与小鼠肌肉细胞融合时，肝细胞高尔基体从其通常的极性位置转变为横纹肌典型的均匀核周位置。人类肝脏白蛋白停止表达，并且在没有DNA复制或细胞分裂的情况下诱导了人类肌肉细胞表面抗原5.1H11的表达。很少观察到肝脏和肌肉蛋白的共表达。这些新发现为基因表达的调控以及在细胞极性、细胞内运输和分泌中起核心作用的细胞器的靶向和定位提供了见解。
