Weintraub H, Tapscott S J, Davis R L, Thayer M J, Adam M A, Lassar A B, Miller A D
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5434-8. doi: 10.1073/pnas.86.14.5434.
MyoD is a master regulatory gene for myogenesis. Under the control of a retroviral long terminal repeat, MyoD was expressed in a variety of differentiated cell types by using either a DNA transfection vector or a retrovirus. Expression of muscle-specific proteins was observed in chicken, human, and rat primary fibroblasts and in differentiated melanoma, neuroblastoma, liver, and adipocyte lines. The ability of MyoD to activate muscle genes in a variety of differentiated cell lines suggests that no additional tissue-specific factors other than MyoD are needed to activate the downstream program for terminal muscle differentiation or that, if such factors exist, they are themselves activated by MyoD expression.
肌分化决定因子（MyoD）是肌肉生成的主要调控基因。在逆转录病毒长末端重复序列的控制下，通过使用DNA转染载体或逆转录病毒，MyoD在多种分化细胞类型中得以表达。在鸡、人及大鼠的原代成纤维细胞以及分化的黑色素瘤、神经母细胞瘤、肝脏和脂肪细胞系中均观察到了肌肉特异性蛋白的表达。MyoD在多种分化细胞系中激活肌肉基因的能力表明，除MyoD外，激活终末肌肉分化的下游程序无需其他额外的组织特异性因子，或者说，如果存在此类因子，它们本身会被MyoD的表达所激活。
