Zhao Hong-Qing, Liu Jian, Meng Pan, Yang Hui, Lin Xiao-Yuan, Long Hong-Ping, Yu Xi-Ming, Wang Yu-Hong
Science and Technology Innovation Center/State Key Laboratory Breeding Base of Chinese Medicine Powder and Innovative Medicine,Hunan University of Chinese Medicine Changsha 410208,China.
Central Laboratory,the First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007,China.
Zhongguo Zhong Yao Za Zhi. 2021 Mar;46(5):1205-1210. doi: 10.19540/j.cnki.cjcmm.20201221.401.
To explore the effect of Baihe Dihuang Decoction on the synaptic plasticity of hippocampal neurons in rats with anxious depression. Fifty SD rats were randomly divided into normal group, model group, venlafaxine group(6.75 mg·kg(-1)), high-dose Baihe Dihuang Decoction group(8.64 g·kg(-1)) and low-dose Baihe Dihuang Decoction group(4.32 g·kg(-1)). Chronic restraint stress(6 h) combined with corticosterone(ih, 30 mg·kg(-1)) was used to establish an anxious depression model, and 7 days after modeling, the administration started and continued for 21 days. The anxiety and depression-like behaviors of the rats were evaluated. Golgi-Cox staining and electron microscopy were used to observe the morphology and ultrastructural changes of synaptic dendrites. Immunofluorescence was used to detect the expression of hippocampal synaptic plasticity protein synapsin-1 and postsynaptic density protein 95(PSD-95). Western blot method was used to detect the expression of functional protein synaptophysin(SYP) and synaptic Ras GTPase activating protein(SynGap). The results showed that the rats in the model group had obvious anxiety and depression-like behaviors, the hip-pocampal dendritic spine density and branch length were reduced, the number of synapses was cut, and the internal structure was da-maged. The average fluorescence intensity of synapsin-1 and PSD-95 was significantly reduced and the expression of SYP and SynGap also decreased. High-dose Baihe Dihuang Decoction could significantly improve the anxiety and depression-like behaviors of model rats, relieve synaptic damage, and increase the expression of synapsin-1, PSD-95, SYP, and SynGap proteins. Therefore, we believe that Baihe Dihuang Decoction can improve anxiety and depression behaviors by regulating the synaptic plasticity of hippocampal neurons.
探讨百合地黄汤对焦虑抑郁模型大鼠海马神经元突触可塑性的影响。将50只SD大鼠随机分为正常组、模型组、文拉法辛组（6.75 mg·kg⁻¹）、百合地黄汤高剂量组（8.64 g·kg⁻¹）和百合地黄汤低剂量组（4.32 g·kg⁻¹）。采用慢性束缚应激（6小时）联合皮质酮（腹腔注射，30 mg·kg⁻¹）建立焦虑抑郁模型，造模7天后开始给药，持续21天。评估大鼠的焦虑和抑郁样行为。采用高尔基-考克斯染色和电子显微镜观察突触树突的形态和超微结构变化。采用免疫荧光法检测海马突触可塑性蛋白突触素-1和突触后致密蛋白95（PSD-95）的表达。采用蛋白质免疫印迹法检测功能性蛋白突触素（SYP）和突触Ras GTP酶激活蛋白（SynGap）的表达。结果显示，模型组大鼠出现明显的焦虑和抑郁样行为，海马树突棘密度和分支长度减少，突触数量减少，内部结构受损。突触素-1和PSD-95的平均荧光强度显著降低，SYP和SynGap的表达也降低。百合地黄汤高剂量组可显著改善模型大鼠的焦虑和抑郁样行为，减轻突触损伤，并增加突触素-1、PSD-95、SYP和SynGap蛋白的表达。因此，我们认为百合地黄汤可通过调节海马神经元的突触可塑性来改善焦虑和抑郁行为。
