Guo Rui, Qin Wei-Shuai, Zhang Shuang-Yong, Zhang Shu-Ling
Department of Encephalopathy, Zhengzhou Chinese Medicine Hospital, Zhengzhou 450007.
Department of Neurology, Zhengzhou People's Hospital, Zhengzhou 450003, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Sep;36(5):444-448. doi: 10.12047/j.cjap.5939.2020.094.
To investigate the effects of Ganmai Dazao Tang on behavior and monoamine neurotransmitters in rats with depression, and to explore its potential mechanism from synaptic structure. Sixty SD rats were randomly divided into 5 groups: normal control group, model group, fluoxetine group (10.8 mg/kg), Ganmai Dazao Tang high and low dose group (9.72, 4.86 g/kg), 12 rats in each group. Except the control group, the rats in the other groups were all chronically unpredictable mild stress (CUMS) to establish a depression model, and were treated by intragastric administration for 21 days. The depression-like behaviors of rats were evaluated by sucrose consumption test and open field test. The contents of serotonin (5-HT) and norepinephrine (NE) in hippocampus were detected by ELISA. The synaptic damage of neurons was observed by Golgi staining. The synaptic structure protein expression levels of MAP-2 and GAP-43 of hippocampus were detected by Immunohistochemistry and Western blot. Compared with control group, the sucrose preference and autonomic activity scores of the depression model rats were decreased significantly (＜0.01), the levels of 5-HT and NE in hippocampus were decreased significantly (＜0.01), and the dendritic spines were absent, and the expressions of MAP-2 and GAP-43 were down-regulated significantly (＜0.01). After treated with Ganmai Dazao Tang, the depression-like behavior of the model rats was significantly relieved (＜0.01), and the levels of 5-HT and NE were increased (＜ 0.05). Dendritic spine density, length and branching were increased, the expressions of MAP-2 and GAP-43 were increased (＜ 0.01). Ganmai Dazao Tang can improve the depression-like behavior of depression model rats and increase the monoamine neurotransmitter content in hippocampus, which may be related to up-regulation of synaptic structural proteins and relief of synaptic damage in neurons.
为探讨甘麦大枣汤对抑郁模型大鼠行为及单胺类神经递质的影响，并从突触结构方面探讨其潜在作用机制。将60只SD大鼠随机分为5组：正常对照组、模型组、氟西汀组（10.8 mg/kg）、甘麦大枣汤高、低剂量组（9.72、4.86 g/kg），每组12只。除正常对照组外，其余各组大鼠均采用慢性不可预知温和应激（CUMS）法建立抑郁模型，并灌胃给药21天。采用蔗糖消耗试验和旷场试验评价大鼠的抑郁样行为。采用ELISA法检测海马组织中5-羟色胺（5-HT）和去甲肾上腺素（NE）含量。采用高尔基染色观察神经元突触损伤情况。采用免疫组织化学和蛋白质免疫印迹法检测海马组织中微管相关蛋白2（MAP-2）和生长相关蛋白43（GAP-43）的突触结构蛋白表达水平。与正常对照组比较，抑郁模型大鼠蔗糖偏好率和自主活动得分显著降低（＜0.01），海马组织中5-HT、NE含量显著降低（＜0.01），树突棘缺失，MAP-2、GAP-43表达显著下调（＜0.01）。甘麦大枣汤干预后，模型大鼠抑郁样行为明显减轻（＜0.01），5-HT、NE含量升高（＜0.05），树突棘密度、长度及分支增加，MAP-2、GAP-43表达增加（＜0.01）。甘麦大枣汤可改善抑郁模型大鼠的抑郁样行为，增加海马组织中单胺类神经递质含量，其机制可能与上调突触结构蛋白表达、减轻神经元突触损伤有关。
