Chen Pan-Pan, Guo Qiao-Sheng, Shi Hong-Zhuan, Zhang Hui-Ning
Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.
Zhongguo Zhong Yao Za Zhi. 2021 Mar;46(6):1374-1378. doi: 10.19540/j.cnki.cjcmm.20201224.102.
Protein kinase C(PKC) is a kind of kinase which is widely involved in cell proliferation and development. PKC(Wp-PKC) in Whitmania pigra body belongs to classic PKC. In order to investigate the effect of Wp-PKC on the development of Wh. pigra germ cells, 17β-estradiol(17β-E2)(100 ng·mL(-1)) and methyltestosterone(MT)(150 μg·L(-1)), 150 μg·L(-1)(MT)+0.5 mg·L(-1) PKC, 0.5 mg·L~(-1) PKC inhibitor were added to Wh. pigra culture water, and no addition group(control group) was added, and the effects on the development of Wh. pigra germ cells and the expression of Wp-PKC were observed. The results showed that: Wp-PKC in male gonads was always higher than that in female gonads; MT promoted the development of male gonads in Wh. pigra, while the expression of Wp-PKC was significantly higher than that in the control; 17β-E2 promoted the development of female gonads in Wh. pigra and Wp-PKC expression significantly lower than that of the control; while the development of the female and male gonads in the PKC inhibitor group was inhibited, the expression of Wp-PKC was significantly lower than that of the control. In summary, Wp-PKC may promote the development of Wh. pigra, especially the development of male gonads.
蛋白激酶C（PKC）是一种广泛参与细胞增殖和发育的激酶。日本医蛭体内的PKC（Wp-PKC）属于经典PKC。为了研究Wp-PKC对日本医蛭生殖细胞发育的影响，在日本医蛭养殖水中添加17β-雌二醇（17β-E2）（100 ng·mL⁻¹）和甲基睾酮（MT）（150 μg·L⁻¹）、150 μg·L⁻¹（MT）+0.5 mg·L⁻¹ PKC、0.5 mg·L⁻¹ PKC抑制剂，不添加任何物质作为空白对照组（对照组），观察其对日本医蛭生殖细胞发育及Wp-PKC表达的影响。结果显示：雄性性腺中的Wp-PKC始终高于雌性性腺；MT促进了日本医蛭雄性性腺的发育，同时Wp-PKC的表达显著高于对照组；17β-E2促进了日本医蛭雌性性腺的发育且Wp-PKC表达显著低于对照组；而PKC抑制剂组的雌性和雄性性腺发育均受到抑制，Wp-PKC的表达显著低于对照组。综上所述，Wp-PKC可能促进日本医蛭的发育，尤其是雄性性腺的发育。
