Zhang Hui-Ning, Guo Qiao-Sheng, Shi Hong-Zhuan, Chen Pan-Pan, Xuan Han-Mei, Yang Pei-Fang, Guo Li-Yuan
Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2020 Apr;45(8):1873-1878. doi: 10.19540/j.cnki.cjcmm.20200205.110.
The present study was conducted to explore the effect of constant temperature overwintering on the growth, gonadal development and internal quality of the Wh. pigra which were in overwintering period. Wh. pigra which in overwintering period were placed in light incubator at 21 ℃, and the Wh. pigra that overwintered under natural conditions were used as control. That the changes of growth performance, gonad index, internal quality of two groups of Wh. pigra were measured at the end of the overwintering. Simultaneously the tissue slice technique was used to observe the morphological structure of the spermary and ovary of the two groups. The results showed that the body weight of constant temperature overwintering Wh. pigra which were placed in light incubator at 21 ℃ was 2.35 times that of natural overwintering Wh. pigra which overwintered under natural conditions, and the weight of female gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 11.54% higher than that of Wh. pigra which overwintered under natural conditions, and the weight of male gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 48.33% higher than that of the Wh. pigra which overwintered under natural conditions. At the same time, that vitellogenesis cells and film forming cells which in ovarian vesicles were significantly higher than those of the Wh. pigra which overwintered under natural conditions, and that spermatogonia and primary spermatocytes which in seminal vesicle were significantly higher than those of Wh. pigra which overwintered under natural conditions. Most important of all, the anti-thrombin activity of Wh. pigra which were placed in light incubator at 21 ℃ increased by 27.85% compared with the Wh. pigra that overwintered under natural conditions. In conclusion, that constant temperature can promote the growth, the development of sperm and egg cells, and the increase of anti-thrombin activity of Wh. pigra which were in overwintering period.
本研究旨在探讨恒温越冬对处于越冬期的白花蛇生长、性腺发育及内在品质的影响。将处于越冬期的白花蛇置于21℃的光照培养箱中进行恒温越冬，以自然条件下越冬的白花蛇作为对照。在越冬结束时测定两组白花蛇的生长性能、性腺指数、内在品质的变化。同时采用组织切片技术观察两组白花蛇精巢和卵巢的形态结构。结果显示，置于21℃光照培养箱中恒温越冬的白花蛇体重是自然条件下越冬白花蛇的2.35倍，置于21℃光照培养箱中的白花蛇雌性性腺重量比自然条件下越冬的白花蛇高11.54%，置于21℃光照培养箱中的白花蛇雄性性腺重量比自然条件下越冬的白花蛇高48.33%。同时，卵巢泡中的卵黄生成细胞和成膜细胞明显高于自然条件下越冬的白花蛇，精囊中精原细胞和初级精母细胞明显高于自然条件下越冬的白花蛇。最重要的是，置于21℃光照培养箱中的白花蛇抗凝血酶活性比自然条件下越冬的白花蛇提高了27.85%。综上所述，恒温可促进处于越冬期的白花蛇生长、精卵细胞发育及抗凝血酶活性增加。
