Li Qian-Hui, Ge Zhuo-Wang, Tian Ding, Xiang Yin, Chen Yu, Zhang Ya-Chen
Department of Geriatrics,Zhongda Hospital,Southeast University Nanjing 210009,China Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University Shanghai 200092,China.
Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University Shanghai 200092,China.
Zhongguo Zhong Yao Za Zhi. 2021 Mar;46(6):1460-1466. doi: 10.19540/j.cnki.cjcmm.20201123.401.
This project aimed to explore the protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation(H/R)-induced H9 c2 cardiomyocyte injury and its underlying signaling pathway. The H/R model of H9 c2 cardiomyocytes was established and then the cells were divided into different treatment groups. CCK-8(cell counting kit-8) was used to detect the activity of cardiomyocytes; Brdu assay was used to detect the proliferation of H9 c2 cells; the caspase-3 activity was tested, and then the protein expression was assessed by Western blot. Flow cytometry was used to evaluate the apoptosis level of cardiomyocytes. Ginsenoside Rg_1 inhibited H/R-induced cardiomyocyte apoptosis and caspase-3 activity, promoted nuclear transcription of nuclear factor erythroid-2 related factor 2(Nrf2), and enhanced the expression of the downstream heme oxygenase-1(HO-1). Ginsenoside Rg_1 could increase Nrf2 nuclear transcription and HO-1 expression with the increase of concentration(10, 20, 40, 60 μmol·L~(-1)). However, the protective effect of ginsenoside Rg_1 on cardiomyocytes was significantly weakened after the transfection of Nrf2-siRNA. Ginsenoside Rg_1 could protect cardiomyocytes by activating the Nrf2/HO-1 pathway.
本项目旨在探讨人参皂苷Rg_1对缺氧/复氧（H/R）诱导的H9 c2心肌细胞损伤的保护作用及其潜在信号通路。建立H9 c2心肌细胞的H/R模型，然后将细胞分为不同处理组。采用CCK-8（细胞计数试剂盒-8）检测心肌细胞活性；采用Brdu检测法检测H9 c2细胞增殖；检测caspase-3活性，然后通过蛋白质印迹法评估蛋白质表达。采用流式细胞术评估心肌细胞凋亡水平。人参皂苷Rg_1抑制H/R诱导的心肌细胞凋亡和caspase-3活性，促进核因子红细胞2相关因子2（Nrf2）的核转录，并增强下游血红素加氧酶-1（HO-1）的表达。人参皂苷Rg_1可随浓度（10、20、40、60 μmol·L~(-1)）增加而增加Nrf2核转录和HO-1表达。然而，转染Nrf2-siRNA后人参皂苷Rg_1对心肌细胞的保护作用明显减弱。人参皂苷Rg_1可通过激活Nrf2/HO-1通路保护心肌细胞。
