Lyu Zhi-Zhen, Zhu Qing-Guang, Kong Ling-Jun, Cheng Yan-Bin, Guo Guang-Xin, Zhou Xin, Zhang Shuai-Pan, Fang Min
Yueyang Hospital of Integrated Trditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhongguo Gu Shang. 2021 Mar 25;34(3):282-7. doi: 10.12200/j.issn.1003-0034.2021.03.018.
To observe the analgesic effect of manipulation loading on chronic low back pain (CLBP) model rats and the expression of inflammatory factors in psoas major muscle tissue, and to explore the improvement of manipulation on local inflammatory microenvironment.
Thirty two SPF male SD rats weighing 340-360g were randomly divided into blank group, sham operation group, chronic low back pain model group and treatment group, with 8 rats in each group. In the model group, L-L lumbar vertebrae were implanted with external link fixation system (ELFS). After implantation of ELFS, the treatment group received manualintervention with 5N force and 2Hz frequency on both sides of the spine, 15 min / time, once a day, for 14 consecutive days. Paw with drawl threshold (PWT) and paw withdrawl latency (PWL) was measured before modeling and on the 1st, 3rd, 7th, 10th and 14th day after intervention. At the end of the treatment cycle, the concentrations of calcitonin gene-related peptide (CGRP) and nerve growth factor (NGF) in psoas muscle were detected by enzyme-linked immunosorbent assay (ELISA).
There was no significant difference in PWT and PWL between the blank group and the sham operation group after modeling (>0.05);after modeling, PWT and PWL in the CLBP model group and the treatment group were significantly decreased(<0.01);PWT in the treatment group was not significantly improved than that in the CLBP model group on the 1st and 3rd day after manual loading(>0.05);on the 7th day after manual loading, the pain threshold value in the treatment group was higher than that in the CLBP model group, but there was no significant difference There was no significant difference between the two groups (=0.056>0.05). On the 10th and 14th day of treatment, the mechanical pain threshold of the treatment group began to rise, and it was statistically significant compared with CLBP model rats (<0.05, < 0.01);on the 1st and 3rd day after manual treatment, the PWL of the treatment group was not significantly improved compared with CLBP model group (>0.05);on the 7th day, the PWL of the treatment group was significantly higher than that of CLBP model group, there was statistical significance (=0.016<0.05). Manual loading improved thermal hyperalgesia in CLBP rats until the end of the experiment. The contents of CGRP and NGF in psoas major muscle of CLBP model group were higher than those of blank group and sham operation group (<0.01). After treatment, the contents of CGRP and NGF decreased significantly(<0.01).
Local massage loading has analgesic effect on CLBP rats, at the same time, it can inhibit the content of CGRP and NGF in psoas muscle tissue of CLBP rats, and improve the local inflammatory microenvironment.
观察手法加载对慢性下腰痛（CLBP）模型大鼠的镇痛效果及腰大肌组织中炎症因子的表达，探讨手法对局部炎症微环境的改善作用。
将32只体重340 - 360g的SPF级雄性SD大鼠随机分为空白组、假手术组、慢性下腰痛模型组和治疗组，每组8只。模型组于L₁ - L₂腰椎植入外固定连接系统（ELFS）。植入ELFS后，治疗组在脊柱两侧以5N的力、2Hz的频率进行手法干预，每次15分钟，每天1次，连续14天。在建模前及干预后第1、3、7、10和14天测量大鼠的爪部缩足阈值（PWT）和爪部缩足潜伏期（PWL）。在治疗周期结束时，采用酶联免疫吸附测定（ELISA）法检测腰大肌中降钙素基因相关肽（CGRP）和神经生长因子（NGF）的浓度。
建模后空白组与假手术组的PWT和PWL比较，差异无统计学意义（>0.05）；建模后，CLBP模型组和治疗组的PWT和PWL均显著降低（<0.01）；手法加载后第1天和第3天，治疗组的PWT与CLBP模型组比较，差异无统计学意义（>0.05）；手法加载后第7天，治疗组的疼痛阈值高于CLBP模型组，但两组间差异无统计学意义（=0.056>0.05）。治疗第10天和第14天，治疗组的机械性疼痛阈值开始升高，与CLBP模型大鼠比较，差异有统计学意义（<0.05，<0.01）；手法治疗后第1天和第3天，治疗组的PWL与CLBP模型组比较，差异无统计学意义（>0.05）；第7天，治疗组的PWL显著高于CLBP模型组，差异有统计学意义（=0.016<0.05）。手法加载可改善CLBP大鼠的热痛觉过敏，直至实验结束。CLBP模型组腰大肌中CGRP和NGF的含量高于空白组和假手术组（<0.01）。治疗后，CGRP和NGF的含量显著降低（<0.01）。
局部按摩加载对CLBP大鼠有镇痛作用，同时可抑制CLBP大鼠腰大肌组织中CGRP和NGF的含量，改善局部炎症微环境。
