Federal Research Center Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2021 Mar;170(5):627-630. doi: 10.1007/s10517-021-05120-8. Epub 2021 Mar 31.
Effects of acute treatment with antidepressant drugs, imipramine and citalopram, on behavior and activity of striatal-enriched tyrosine protein phosphatase (STEP) in the whole brain of zebrafish Danio rerio were studied. Mature zebrafish were exposed for 3 h to water (control) or to solutions of 0.25, 0.5, or 1 mg/liter of imipramine or citalopram, and then their behavior was studied in novel tank test. STEP activity was assayed in the brain of animals by the difference between the rates of transformation of p-nitrophenyl phosphate to 4-nitrophenol in the absence or presence of a selective STEP inhibitor. In novel tank test, imipramine and citalopram reduced locomotor activity and increased freezing time; at this, imipramine increased the total time spent in top of the tank. Citalopram (all concentrations) and imipramine (0.5 and 1 mg/liter) increased STEP activity in zebrafish brain.
研究了抗抑郁药丙咪嗪和西酞普兰对斑马鱼大脑中富含多巴胺的酪氨酸蛋白磷酸酶（STEP）的行为和活性的急性作用。将成熟的斑马鱼暴露于水中（对照）或 0.25、0.5 或 1mg/L 的丙咪嗪或西酞普兰溶液中 3 小时，然后在新的鱼缸测试中研究其行为。通过比较在不存在或存在选择性 STEP 抑制剂的情况下，p-硝基苯磷酸转化为 4-硝基苯酚的速率，在动物的大脑中测定 STEP 活性。在新鱼缸测试中，丙咪嗪和西酞普兰降低了运动活性并增加了冻结时间；在此过程中，丙咪嗪增加了在鱼缸顶部的总停留时间。西酞普兰（所有浓度）和丙咪嗪（0.5 和 1mg/L）增加了斑马鱼大脑中的 STEP 活性。
