通过 miR-205-5p 和促甲状腺激素受体 mRNA 区分良性甲状腺结节和恶性甲状腺结节。

Differentiation of benign thyroid nodules from malignant thyroid nodules through miR-205-5p and thyroid-stimulating hormone receptor mRNA.

机构信息

Department of Breast and Thyroid Surgery, Yiyang Central Hospital, No.108 Kangfu North Road, Yiyang, 413000, People's Republic of China.

Department of General Surgery, Yiyang Central Hospital, No. 108 Kangfu North Road, Yiyang, 413000, People's Republic of China.

出版信息

Hormones (Athens). 2021 Sep;20(3):571-580. doi: 10.1007/s42000-021-00286-2. Epub 2021 Mar 31.

DOI:10.1007/s42000-021-00286-2

PMID:33788150

Abstract

PURPOSE

This study was designed to determine the value of miR-205-5p and thyroid-stimulating hormone receptor (TSHR) mRNA in differentiating benign thyroid nodules from malignant thyroid nodules.

METHODS

A total of 67 patients with malignant thyroid nodules admitted to our hospital from October 2016 to March 2018 were enrolled in the malignant group, and 71 patients with benign thyroid nodules were enrolled in the benign group. Another 56 healthy individuals, as determined by physical examination, were enrolled in the control group. Quantitative real-time polymerase chain reaction (qRT-PCR) was adopted to determine the relative expression of miR-205-5p and TSHR mRNA in serum, and receiver operating characteristic (ROC) curves were used to analyze the diagnostic value of miR-205-5p and TSHR mRNA in thyroid nodules and their benignancy or malignancy. The correlation of miR-205-5p and TSHR mRNA with clinical data of the patients with thyroid carcinoma was analyzed, and multivariate logistic regression was used to analyze risk factors for lymph node metastasis of thyroid carcinoma. In addition, Pearson's test was used to analyze the relationship between miR-205-5p and TSHR mRNA in serum of patients with thyroid carcinoma.

RESULTS

The malignant group showed significantly higher expression of miR-205-5p and TSHR mRNA than the benign group and control group (both p <0.001), and the benign group showed significantly higher expression of these than the control group (p <0.01). miR-205-5p expression was related to tumor size, TNM staging, lymph node metastasis, capsular infiltration, and BRAF mutation (p <0.05), and TSHR mRNA expression was related to lymph node metastasis, capsular infiltration, BRAF mutation, and RAS mutation (p <0.05). Gender, number of lesions, capsular infiltration, miR-205-5p, and TSHR mRNA were independent risk factors for lymph node metastasis of patients with thyroid carcinoma. Moreover, there was a positive correlation between miR-205-5p and TSHR mRNA in patients with thyroid carcinoma (r=0.735, p<0.001).

CONCLUSION

MiR-205-5p and TSHR mRNA may be potential diagnosis indexes of thyroid nodules, their benignancy, or malignancy, while the independent risk factors for patients with thyroid carcinoma include a large number of lesions, occurrence of capsular infiltration, and relatively high expression of miR-205-5p and TSHR mRNA.

摘要

目的

本研究旨在探讨微小 RNA-205-5p（miR-205-5p）和促甲状腺激素受体（TSHR）mRNA 在鉴别甲状腺良恶性结节中的价值。

方法

选取 2016 年 10 月至 2018 年 3 月我院收治的 67 例甲状腺恶性结节患者为恶性组，71 例甲状腺良性结节患者为良性组，另选取同期健康体检者 56 例为对照组。采用实时荧光定量聚合酶链反应（qRT-PCR）检测血清中 miR-205-5p 和 TSHR mRNA 的相对表达水平，采用受试者工作特征（ROC）曲线分析 miR-205-5p 和 TSHR mRNA 对甲状腺结节良恶性的诊断价值。分析甲状腺癌患者临床资料与 miR-205-5p 和 TSHR mRNA 的相关性，采用多因素 logistic 回归分析甲状腺癌淋巴结转移的危险因素。此外，采用 Pearson 检验分析甲状腺癌患者血清中 miR-205-5p 和 TSHR mRNA 的相关性。

结果

恶性组 miR-205-5p 和 TSHR mRNA 的表达水平显著高于良性组和对照组（均 P<0.001），良性组显著高于对照组（P<0.01）。miR-205-5p 表达与肿瘤大小、TNM 分期、淋巴结转移、包膜浸润和 BRAF 突变有关（P<0.05），TSHR mRNA 表达与淋巴结转移、包膜浸润、BRAF 突变和 RAS 突变有关（P<0.05）。性别、病灶数量、包膜浸润、miR-205-5p 和 TSHR mRNA 是甲状腺癌患者淋巴结转移的独立危险因素。此外，甲状腺癌患者 miR-205-5p 和 TSHR mRNA 呈正相关（r=0.735，P<0.001）。