Bogaczyk Anna, Potocka Natalia, Paszek Sylwia, Skrzypa Marzena, Zuchowska Alina, Kośny Michał, Kluz-Barłowska Marta, Wróbel Andrzej, Wróbel Jan, Zawlik Izabela, Kluz Tomasz
Department of Gynecology, Gynecology Oncology and Obstetrics, Fryderyk Chopin University Hospital, 35-055 Rzeszow, Poland.
Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, Collegium Medicum, University of Rzeszow, 35-959 Rzeszow, Poland.
Int J Mol Sci. 2025 Mar 14;26(6):2615. doi: 10.3390/ijms26062615.
Endometrial cancer is the fourth most common cancer in women in Europe. Its carcinogenesis is a complex process and requires further research. In our study, we focus on finding new and easy-to-diagnose markers for detecting endometrial cancer. For this purpose, we compared the levels of miR-21-5p, miR-205-5p, and miR-222-3p in endometrial cancer tissues with the levels of these miRs in the serum of patients using the dPCR method. Our study is preliminary and consists of comparing the changes in miRNA expression in serum to the changes in miRNA in tissue of patients with endometrial cancer. The study included 18 patients with EC and 19 patients undergoing surgery for pelvic organ prolapse or uterine fibroids as a control group without neoplastic lesions. Endometrial tissue and serum were collected from all patients. The analyses showed an increased expression of miR-205-5p in endometrial cancer tissue and decreased expression of miR-222-3p in tissue and serum samples. These results suggest that miR-205-5p and miR-222-3p may be potential endometrial cancer biomarkers. Only miR-222-3p confirmed its decreased expression in serum, making it a potential and easily accessible marker in the diagnosis of endometrial cancer. This pilot study requires further investigation in a larger group of patients. Its advantages include the possibility of a comparison between miRNA expression in tissue and serum, as well as conducting the study using dPCR.
子宫内膜癌是欧洲女性中第四大常见癌症。其致癌过程是一个复杂的过程，需要进一步研究。在我们的研究中，我们专注于寻找用于检测子宫内膜癌的新的且易于诊断的标志物。为此，我们使用数字PCR方法比较了子宫内膜癌组织中miR-21-5p、miR-205-5p和miR-222-3p的水平与患者血清中这些miR的水平。我们的研究是初步的，包括比较血清中miRNA表达的变化与子宫内膜癌患者组织中miRNA的变化。该研究纳入了18例子宫内膜癌患者和19例因盆腔器官脱垂或子宫肌瘤接受手术的患者作为无肿瘤病变的对照组。从所有患者中收集子宫内膜组织和血清。分析显示，miR-205-5p在子宫内膜癌组织中的表达增加，而miR-222-3p在组织和血清样本中的表达降低。这些结果表明，miR-205-5p和miR-222-3p可能是潜在的子宫内膜癌生物标志物。只有miR-222-3p在血清中证实了其表达降低，使其成为子宫内膜癌诊断中一种潜在且易于获取的标志物。这项初步研究需要在更大规模的患者群体中进一步研究。其优点包括能够比较组织和血清中miRNA的表达，以及使用数字PCR进行研究。
