柚皮素和姜黄素对 HeLa 细胞凋亡、副凋亡和自噬相关细胞死亡的协同作用。

Synergistic Effect of Apigenin and Curcumin on Apoptosis, Paraptosis and Autophagy-related Cell Death in HeLa Cells.

机构信息

Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.

Department of Chemistry-Biology Engineering, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul, Turkey.

出版信息

Anticancer Res. 2021 Mar;41(3):1271-1282. doi: 10.21873/anticanres.14884.

DOI:10.21873/anticanres.14884

PMID:33788718

Abstract

BACKGROUND/AIM: We aimed to investigate the synergistic effects of apigenin and curcumin on the cross-talk between apoptosis and autophagic cell death, as well as on paraptosis in HeLa cells.

MATERIALS AND METHODS

Cell viability was measured using the MTT assay. Synergistic effects were measured using the Bliss independence model. qRT-PCR was used to study the expression of genes related to apoptosis, autophagic cell death, and cross-talk. GRP78/BiP immunostaining was used to identify endoplasmic reticulum (ER) stress.

RESULTS

Treatment with a combination of apigenin and curcumin increased the expression levels of genes related to cell death in HeLa cells 1.29- to 27.6-fold. The combination of curcumin and apigenin showed a synergistic anti-tumor effect via cross-talk between processes leading to apoptosis and autophagic cell death, as well as ER stress-associated paraptosis. GRP78 expression was down-regulated, and massive cytoplasmic vacuolization was observed in HeLa cells.

CONCLUSION

The combination of curcumin and apigenin is an effective potential therapeutic for cervical cancers.

摘要

背景/目的：我们旨在研究芹菜素和姜黄素在 HeLa 细胞中凋亡和自噬性细胞死亡的串扰以及副凋亡之间的协同作用。

材料和方法

使用 MTT 测定法测量细胞活力。使用 Bliss 独立性模型测量协同作用。qRT-PCR 用于研究与凋亡、自噬性细胞死亡和串扰相关的基因表达。GRP78/BiP 免疫染色用于鉴定内质网 (ER) 应激。

结果

用芹菜素和姜黄素联合处理可使 HeLa 细胞中与细胞死亡相关的基因表达水平增加 1.29 至 27.6 倍。姜黄素和芹菜素的联合作用通过诱导凋亡和自噬性细胞死亡以及与内质网应激相关的副凋亡的过程之间的串扰表现出协同的抗肿瘤作用。GRP78 的表达下调，并且在 HeLa 细胞中观察到大量细胞质空泡化。