Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand.
Pharm Biol. 2021 Dec;59(1):367-374. doi: 10.1080/13880209.2021.1893348.
ECa 233 is the standardized extract of (L.) Urban. (Apiaceae). It contains at least 85% of triterpenoid glycosides and yields neuroprotective and memory-enhancing effects. However, the exact molecules exerting the effects might be triterpenic acid metabolites reproduced through gut metabolism after orally ingesting , not triterpenoid glycosides.
This study demonstrates the effect of unmetabolized ECa 233 on hippocampal synaptic plasticity after directly perfusing ECa 233 over acute brain slices.
The brain slices obtained from 7-week-old male Wistar rats were randomly divided into 4 groups. We perfused either artificial cerebrospinal fluid (ACSF), 0.01% DMSO, 10 µg/mL ECa 233, or 100 µg/mL on brain slices, and measured the long-term potentiation (LTP) magnitude to determine the synaptic plasticity of hippocampal circuits in each group.
The LTP magnitude of ACSF, DMSO, 10 ug/mL ECa 233, and 100 ug/mL ECa 233 groups increased from 100% to 181.26 ± 38.19%, 148.74 ± 5.40%, 273.71 ± 56.66%, 182.17 ± 18.61%, respectively. ECa 233 at the concentration of 10 µg/mL robustly and significantly enhanced hippocampal LTP magnitude. The data indicates an improvement of the hippocampal synaptic plasticity.
This study emphasizes the effectiveness of triterpenoid glycosides in ECa 233 on synaptic plasticity enhancement. Therefore, this study supported and complimented the known effects of extract on the enhancement of synaptic plasticity, and subsequently, learning and memory, suggesting that ECa 233 could be a promising memory enhancing agent.
ECa 233 是标准化提取物（Urban）。它含有至少 85%的三萜糖苷，并具有神经保护和增强记忆的作用。然而，确切的发挥作用的分子可能是通过口服摄入后肠道代谢产生的三萜酸代谢物，而不是三萜糖苷。
本研究通过在急性脑片中直接灌注 ECa 233，证明未代谢的 ECa 233 对海马突触可塑性的影响。
从 7 周龄雄性 Wistar 大鼠获得的脑片随机分为 4 组。我们分别用人工脑脊液（ACSF）、0.01%DMSO、10μg/ml ECa 233 或 100μg/ml 灌注脑片，并测量长时程增强（LTP）幅度，以确定每组海马回路的突触可塑性。
ACSF、DMSO、10μg/ml ECa 233 和 100μg/ml ECa 233 组的 LTP 幅度从 100%增加到 181.26±38.19%、148.74±5.40%、273.71±56.66%和 182.17±18.61%。浓度为 10μg/ml 的 ECa 233 可显著增强海马 LTP 幅度。数据表明海马突触可塑性得到改善。
本研究强调了 ECa 233 中三萜糖苷对增强突触可塑性的有效性。因此，本研究支持并补充了提取物对增强突触可塑性以及随后学习和记忆的已知作用，表明 ECa 233 可能是一种有前途的增强记忆的药物。
