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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.过氧化物酶标记抗体及具有增强细胞内穿透能力的Fab缀合物。
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2
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J Gen Microbiol. 1972 Aug;72(1):127-41. doi: 10.1099/00221287-72-1-127.
3
The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.免疫组织化学的未标记抗体酶法：可溶性抗原-抗体复合物（辣根过氧化物酶-抗辣根过氧化物酶）的制备、性质及其在螺旋体鉴定中的应用
J Histochem Cytochem. 1970 May;18(5):315-33. doi: 10.1177/18.5.315.
4
A galactosidase immunosorbent test for carcinoembryonic antigen.一种用于癌胚抗原的半乳糖苷酶免疫吸附试验。
Cancer Res. 1976 Aug;36(8):2845-9.
5
An indirect radiolabelled antibody staining technique for the rapid detection and identification of bacteria.一种用于快速检测和鉴定细菌的间接放射性标记抗体染色技术。
J Appl Bacteriol. 1976 Aug;41(1):47-58. doi: 10.1111/j.1365-2672.1976.tb00604.x.
6
Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.使用间接酶标抗体试验快速筛选针对病原体抗体的微量方法。
J Clin Microbiol. 1976 Jun;3(6):604-8. doi: 10.1128/jcm.3.6.604-608.1976.

通过化学发光免疫反应对少量微生物进行快速鉴定和定量。

Rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction.

作者信息

Halmann M, Velan B, Sery T

出版信息

Appl Environ Microbiol. 1977 Nov;34(5):473-7. doi: 10.1128/aem.34.5.473-477.1977.

DOI:10.1128/aem.34.5.473-477.1977
PMID:337894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242685/
Abstract

A method (patent pending) for rapidly identifying and quantitating small numbers of microorganisms was developed based on the specific immunoreaction of microorganisms with homologous antibodies linked by conjugation to peroxidase. The high sensitivity of the method is due to the use of a chemiluminescent reaction for the determination of the enzyme. The reaction was performed on Alcar supports with low nonspecific adsorption. The very low noise achieved permitted the detection of as few as 30 to 300 bacterial cells.

摘要

基于微生物与通过共轭连接到过氧化物酶的同源抗体的特异性免疫反应，开发了一种（正在申请专利）快速识别和定量少量微生物的方法。该方法的高灵敏度归因于使用化学发光反应来测定酶。反应在具有低非特异性吸附的Alcar载体上进行。所实现的极低噪声允许检测低至30至300个细菌细胞。