通过化学发光免疫反应对少量微生物进行快速鉴定和定量。
Rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction.
作者信息
Halmann M, Velan B, Sery T
出版信息
Appl Environ Microbiol. 1977 Nov;34(5):473-7. doi: 10.1128/aem.34.5.473-477.1977.
Abstract
A method (patent pending) for rapidly identifying and quantitating small numbers of microorganisms was developed based on the specific immunoreaction of microorganisms with homologous antibodies linked by conjugation to peroxidase. The high sensitivity of the method is due to the use of a chemiluminescent reaction for the determination of the enzyme. The reaction was performed on Alcar supports with low nonspecific adsorption. The very low noise achieved permitted the detection of as few as 30 to 300 bacterial cells.
摘要
基于微生物与通过共轭连接到过氧化物酶的同源抗体的特异性免疫反应，开发了一种（正在申请专利）快速识别和定量少量微生物的方法。该方法的高灵敏度归因于使用化学发光反应来测定酶。反应在具有低非特异性吸附的Alcar载体上进行。所实现的极低噪声允许检测低至30至300个细菌细胞。
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