Saunders G C, Clinard E H
J Clin Microbiol. 1976 Jun;3(6):604-8. doi: 10.1128/jcm.3.6.604-608.1976.
We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.
我们开发了一种快速简便的间接酶标抗体检测方法,适用于筛查针对病毒、细菌和寄生虫抗原的抗体。一次性微孔板用作抗原载体和检测工具。该检测方法对血清或未凝固的全血同样有效。使用自动移液器,30至60分钟内可进行96次检测。我们报告了用于动物疾病检测和监测的酶标抗体检测方法的开发情况。