Determination of pools of tricarboxylic acid cycle and related acids in bacteria.
作者信息
Siegel W H, Donohue T, Bernlohr R W
出版信息
Appl Environ Microbiol. 1977 Nov;34(5):512-7. doi: 10.1128/aem.34.5.512-517.1977.
Abstract
Methods for sampling, extracting, and quantitating the metabolic pools of organic acids from bacteria have been developed. The concentration of these metabolites was determined by a new gas chromatographic method that can quantitatively determine the levels of lactate, pyruvate, fumarate, succinate, malate, alpha-ketoglutarate, and citrate. Values obtained were confirmed by fluorimetric analyses of five of the individual acids. In Escherichia coli, pools range from about 1 to 5 mumol/g of dry weight, with a variation in replicate samples of 5 to 15%. Under similar conditions, these pools in Bacillus licheniformis are in the same range, although the pyruvic acid pool is significantly larger.
摘要
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