细菌中三羧酸循环及相关酸的库的测定
Determination of pools of tricarboxylic acid cycle and related acids in bacteria.
作者信息
Siegel W H, Donohue T, Bernlohr R W
出版信息
Appl Environ Microbiol. 1977 Nov;34(5):512-7. doi: 10.1128/aem.34.5.512-517.1977.
Abstract
Methods for sampling, extracting, and quantitating the metabolic pools of organic acids from bacteria have been developed. The concentration of these metabolites was determined by a new gas chromatographic method that can quantitatively determine the levels of lactate, pyruvate, fumarate, succinate, malate, alpha-ketoglutarate, and citrate. Values obtained were confirmed by fluorimetric analyses of five of the individual acids. In Escherichia coli, pools range from about 1 to 5 mumol/g of dry weight, with a variation in replicate samples of 5 to 15%. Under similar conditions, these pools in Bacillus licheniformis are in the same range, although the pyruvic acid pool is significantly larger.
摘要
已开发出从细菌中采样、提取和定量有机酸代谢库的方法。这些代谢物的浓度通过一种新的气相色谱法测定，该方法可定量测定乳酸、丙酮酸、富马酸、琥珀酸、苹果酸、α-酮戊二酸和柠檬酸的水平。所获得的值通过对其中五种单个酸的荧光分析得到确认。在大肠杆菌中，代谢库范围为每克干重约1至5微摩尔，重复样品的变化为5%至15%。在类似条件下，地衣芽孢杆菌中的这些代谢库处于相同范围，尽管丙酮酸代谢库明显更大。
相似文献
1
Determination of pools of tricarboxylic acid cycle and related acids in bacteria.细菌中三羧酸循环及相关酸的库的测定
Appl Environ Microbiol. 1977 Nov;34(5):512-7. doi: 10.1128/aem.34.5.512-517.1977.
2
Separation of lactic acid and some Krebs cycle acids by thin-layer chromatography.
Anal Biochem. 1966 Apr;15(1):122-6. doi: 10.1016/0003-2697(66)90254-5.
3
Gas chromatography of Krebs cycle and related acids on Silar 10C.
Clin Biochem. 1975 Apr;8(2):148-50. doi: 10.1016/s0009-9120(75)91541-6.
4
Separation of intermediates of the citric acid cycle and related compounds by thin-layer chromatography.通过薄层色谱法分离柠檬酸循环中间体及相关化合物。
Anal Biochem. 1966 Nov;17(2):210-3. doi: 10.1016/0003-2697(66)90199-0.
5
Separation and quantitative determination of lactic, pyruvic, fumaric, succinic, malic, and citric acids by gas chromatography.
Anal Biochem. 1969 Jul;30(1):32-9. doi: 10.1016/0003-2697(69)90370-4.
6
High-resolution chromatographic system for measuring organic acids in biological samples.
Clin Chem. 1971 Aug;17(8):721-30.
7
Effects of morphine and pain on brain intermediary metabolism.
Neuropharmacology. 1972 Nov;11(6):871-7. doi: 10.1016/0028-3908(72)90046-9.
8
Separation and determination of 14C-labelled intermediates of the citric acid cycle and related compounds.
Anal Biochem. 1974 May;59(1):110-21. doi: 10.1016/0003-2697(74)90015-3.
9
Isolation of Krebs cycle acids from tissues for gas chromatography.
Anal Biochem. 1967 Jun;19(3):468-80. doi: 10.1016/0003-2697(67)90238-2.
10
Gas chromatographic separation of tricarboxylic acid cycle components.
Clin Chim Acta. 1970 Feb;27(2):233-40. doi: 10.1016/0009-8981(70)90340-2.
引用本文的文献
1
Overcoming fluctuation and leakage problems in the quantification of intracellular 2-oxoglutarate levels in Escherichia coli.克服大肠杆菌细胞内 2-氧戊二酸水平定量中的波动和渗漏问题。
Appl Environ Microbiol. 2011 Oct;77(19):6763-71. doi: 10.1128/AEM.05257-11. Epub 2011 Aug 5.
2
A novel alpha-ketoglutarate reductase activity of the serA-encoded 3-phosphoglycerate dehydrogenase of Escherichia coli K-12 and its possible implications for human 2-hydroxyglutaric aciduria.大肠杆菌K-12中由serA编码的3-磷酸甘油酸脱氢酶的一种新型α-酮戊二酸还原酶活性及其对人类2-羟基戊二酸尿症的可能影响。
J Bacteriol. 1996 Jan;178(1):232-9. doi: 10.1128/jb.178.1.232-239.1996.
3
Purification and properties of glutamate synthase from Bacillus licheniformis.地衣芽孢杆菌谷氨酸合酶的纯化及性质
J Bacteriol. 1984 Nov;160(2):591-9. doi: 10.1128/jb.160.2.591-599.1984.
4
Regulation of nitrogen catabolic enzymes in Bacillus spp.芽孢杆菌属中氮分解代谢酶的调控
J Bacteriol. 1982 Aug;151(2):971-5. doi: 10.1128/jb.151.2.971-975.1982.
5
Changes in free amino acid content and activities of amination and transamination enzymes in yeasts grown on different inorganic nitrogen sources, including hydroxylamine.在包括羟胺在内的不同无机氮源上生长的酵母中游离氨基酸含量以及胺化和转氨作用酶活性的变化。
Antonie Van Leeuwenhoek. 1981;47(3):217-30. doi: 10.1007/BF00403393.
6
Properties of the Bacillus licheniformis A5 glutamine synthetase purified from cells grown in the presence of ammonia or nitrate.从在氨或硝酸盐存在下生长的细胞中纯化得到的地衣芽孢杆菌A5谷氨酰胺合成酶的性质。
J Bacteriol. 1981 Aug;147(2):589-601. doi: 10.1128/jb.147.2.589-601.1981.
7
Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis.β-内酰胺酶抑制剂克拉维酸在地衣芽孢杆菌营养缺陷型突变体分离中的应用。
J Bacteriol. 1984 Aug;159(2):803-4. doi: 10.1128/jb.159.2.803-804.1984.
8
Nutrient-stimulated methylation of a membrane protein in Bacillus licheniformis.地衣芽孢杆菌中营养物质刺激的膜蛋白甲基化作用
J Bacteriol. 1988 Sep;170(9):4113-8. doi: 10.1128/jb.170.9.4113-4118.1988.
9
Expression of alpha-amylase in Bacillus licheniformis.地衣芽孢杆菌中α-淀粉酶的表达
J Bacteriol. 1986 Nov;168(2):839-42. doi: 10.1128/jb.168.2.839-842.1986.
10
Carbon isotopic fractionation in heterotrophic microbial metabolism.异养微生物代谢中的碳同位素分馏
Appl Environ Microbiol. 1985 Oct;50(4):996-1001. doi: 10.1128/aem.50.4.996-1001.1985.
本文引用的文献
1
A gas-liquid-chromatographic procedure for separating a wide range of metabolites occuring in urine or tissue extracts.一种用于分离尿液或组织提取物中广泛存在的代谢物的气-液-色谱法。
Biochem J. 1966 Dec;101(3):792-810. doi: 10.1042/bj1010792.
2
ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中的3',5'-磷酸腺苷
J Biol Chem. 1965 Mar;240:1309-14.
3
Changes in amino acid permeation during sporulation.孢子形成过程中氨基酸渗透的变化。
J Bacteriol. 1967 Mar;93(3):1031-44. doi: 10.1128/jb.93.3.1031-1044.1967.
4
Quantification of seven tricarboxylic acid cycle and related acids in human urine by gas-liquid chromatography.气相色谱法对人尿液中七种三羧酸循环及相关酸的定量分析
Anal Chem. 1969 Nov;41(13):1781-7. doi: 10.1021/ac60282a034.
5
Separation and quantitative determination of lactic, pyruvic, fumaric, succinic, malic, and citric acids by gas chromatography.
Anal Biochem. 1969 Jul;30(1):32-9. doi: 10.1016/0003-2697(69)90370-4.
6
Gas chromatographic separation and determination of microquantities of the esters of the tricarboxylic acid cycle acids and related compounds.
J Chromatogr. 1969 Jun 17;42(2):157-69. doi: 10.1016/s0021-9673(01)80611-7.
7
Characterization and regulation of protease synthesis and activity in Bacillus licheniformis.地衣芽孢杆菌中蛋白酶合成与活性的表征及调控
J Bacteriol. 1971 Jan;105(1):276-83. doi: 10.1128/jb.105.1.276-283.1971.
8
Turnover rates and intracellular pool size distribution of citrate cycle intermediates in normal, diabetic and fat-fed rats estimated by computer analysis from specific activity decay data of 14C-labeled citrate cycle acids.通过对14C标记的柠檬酸循环酸的比活性衰减数据进行计算机分析，估算正常、糖尿病和高脂喂养大鼠中柠檬酸循环中间体的周转率和细胞内池大小分布。
Eur J Biochem. 1970 Dec;17(2):350-63. doi: 10.1111/j.1432-1033.1970.tb01173.x.
9
Gas chromatographic separation of tricarboxylic acid cycle components.
Clin Chim Acta. 1970 Feb;27(2):233-40. doi: 10.1016/0009-8981(70)90340-2.
10
Changes in free amino acid production and intracellular amino acid pools of Bacillus licheniformis as a function of culture age and growth media.地衣芽孢杆菌游离氨基酸产量和细胞内氨基酸库随培养时间和生长培养基的变化。
J Bacteriol. 1972 Nov;112(2):715-25. doi: 10.1128/jb.112.2.715-725.1972.
Zotero 插件