College of Natural Resources and Environment, Northwest A&F University.
Shaanxi Province Institute of Microbiology.
J Gen Appl Microbiol. 2021 Jul 31;67(3):106-113. doi: 10.2323/jgam.2020.08.003. Epub 2021 Mar 31.
A pot experiment was conducted with kiwifruit planting soil to evaluate the impacts of potassium solubilizing bacteria (KSB) and K-feldspar on the soil nutrient levels, enzyme activities, and microecological environment. The effects were investigated of three inoculation treatments (T1: K-feldspar, T2: KSB, and T3: KSB with K-feldspar) and a non-inoculation treatment (CK) on the enzyme activities and the metabolic activities of the bacterial communities in kiwifruit rhizosphere soil. The results showed that the total nitrogen, available phosphorus, available potassium, and organic matter contents in T3 were 18.19%, 45.22%, 15.06%, and 4.17% higher, respectively, than those in CK at the end of the experiment (90 days). Compared with CK, T3 significantly increased the invertase, urease, acid phosphatase, and polyphenol oxidase activities. T3 had a higher kiwifruit root activity, but there were no significant differences among the four treatments (P > 0.05). T3 significantly altered the bacterial community diversity, increased the utilization of phenolic compounds and polymers, and decreased the utilization of amino acids. Redundancy analysis indicated that soil nutrients (total nitrogen, available phosphorus, and available potassium) and enzyme activities (urease and acid phosphatase) had more important effects on the metabolic activities of the bacterial communities. Co-inoculation enhanced the soil nutrients, enzyme activities, and bacterial community diversity. KSB co-inoculated with K-feldspar has the potential to improve the soil fertility, microbial metabolic activity and plant growth.
采用猕猴桃种植土壤进行盆栽试验，评估解钾细菌（KSB）和钾长石对土壤养分水平、酶活性和微生态环境的影响。研究了三种接种处理（T1：钾长石，T2：KSB，T3：KSB 加钾长石）和一种非接种处理（CK）对猕猴桃根际土壤酶活性和细菌群落代谢活性的影响。结果表明，试验结束时（90 天），T3 的全氮、有效磷、速效钾和有机质含量分别比 CK 高 18.19%、45.22%、15.06%和 4.17%。与 CK 相比，T3 显著提高了转化酶、脲酶、酸性磷酸酶和多酚氧化酶活性。T3 具有较高的猕猴桃根活力，但四种处理之间无显著差异（P>0.05）。T3 显著改变了细菌群落多样性，增加了对酚类化合物和聚合物的利用，降低了对氨基酸的利用。冗余分析表明，土壤养分（全氮、有效磷和速效钾）和酶活性（脲酶和酸性磷酸酶）对细菌群落代谢活性的影响更为重要。共接种增强了土壤养分、酶活性和细菌群落多样性。KSB 与钾长石共接种具有提高土壤肥力、微生物代谢活性和植物生长的潜力。
