Li Lirong, Ren Shaohua, Hao Xudong, Zhen Zigang, Ji Lei, Ji Hongming
Department of Neurosurgery of Shanxi Provincial People's Hospital, Taiyuan, Shanxi 030012, P.R. China.
Exp Ther Med. 2021 May;21(5):492. doi: 10.3892/etm.2021.9923. Epub 2021 Mar 17.
Intracranial aneurysms (IAs) are bulges of blood vessels in the cerebral area. The development and progression of IAs are associated with the proliferation of vascular smooth muscle cells (VSMCs) during phenotypic modulation under environmental cues. MicroRNA-29b (miR-29b) has been studied extensively and demonstrated to reduce cell proliferation in various diseases by binding to the 3'-untranslated region (3'-UTR) of a variety of target messenger RNAs (mRNAs), thereby inhibiting their translation. The present study aimed to investigate the role of miR-29b on the proliferation of VSMCs and human umbilical artery smooth muscle cells. The results indicated that the overexpression of miR-29b reduced cell migration and proliferation. Western blotting results indicated that this effect may be attributed to the attenuation of a signaling pathway involving transforming growth factor β (TGF-β) and Smad3 proteins. Luciferase assay confirmed the binding of miR-29b to TGF-β1 and the knockdown of TGF-β1 reduced miR-29b inhibitor-induced cell migration. The present study indicates that miR-29b downregulates the expression of TGF-β1 by targeting the 3'-UTR of its mRNA and modulates cell migration and proliferation via the TGF-β1/Smad3 signaling pathway.
颅内动脉瘤（IAs）是脑区血管的膨出。在环境信号作用下，颅内动脉瘤的发生和发展与血管平滑肌细胞（VSMCs）表型调节过程中的增殖有关。MicroRNA-29b（miR-29b）已被广泛研究，并证明通过与多种靶信使核糖核酸（mRNAs）的3'-非翻译区（3'-UTR）结合来减少各种疾病中的细胞增殖，从而抑制其翻译。本研究旨在探讨miR-29b对VSMCs和人脐动脉平滑肌细胞增殖的作用。结果表明，miR-29b的过表达降低了细胞迁移和增殖。蛋白质印迹结果表明，这种作用可能归因于涉及转化生长因子β（TGF-β）和Smad3蛋白的信号通路的减弱。荧光素酶测定证实了miR-29b与TGF-β1的结合，TGF-β1的敲低减少了miR-29b抑制剂诱导的细胞迁移。本研究表明，miR-29b通过靶向其mRNA的3'-UTR下调TGF-β1的表达，并通过TGF-β1/Smad3信号通路调节细胞迁移和增殖。
