Institut Pasteur, Department of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, CNRS UMR3523, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
Université de Paris, 12 rue de l'École de Médecine, 75006 Paris, France.
Metallomics. 2021 Apr 21;13(4). doi: 10.1093/mtomcs/mfab016.
Artificial metal base pairs have become increasingly important in nucleic acids chemistry due to their high thermal stability, water solubility, orthogonality to natural base pairs, and low cost of production. These interesting properties combined with ease of chemical and enzymatic synthesis have prompted their use in several practical applications, including the construction of nanomolecular devices, ions sensors, and metal nanowires. Chemical synthesis of metal base pairs is highly efficient and enables the rapid screening of novel metal base pair candidates. However, chemical synthesis is limited to rather short oligonucleotides and requires rather important synthetic efforts. Herein, we discuss recent progress made for the enzymatic construction of metal base pairs that can alleviate some of these limitations. First, we highlight the possibility of generating metal base pairs using canonical nucleotides and then describe how modified nucleotides can be used in this context. We also provide a description of the main analytical techniques used for the analysis of the nature and the formation of metal base pairs together with relevant examples of their applications.
人工金属碱基对由于其高热稳定性、水溶性、与天然碱基对的正交性以及低成本的生产而在核酸化学中变得越来越重要。这些有趣的性质加上易于化学和酶合成，促使它们在几个实际应用中得到了应用，包括构建纳米分子器件、离子传感器和金属纳米线。金属碱基对的化学合成效率很高，能够快速筛选新型金属碱基对候选物。然而，化学合成仅限于相当短的寡核苷酸，并且需要相当大的合成努力。在此，我们讨论了酶促构建金属碱基对的最新进展，这些碱基对可以缓解其中的一些限制。首先，我们强调了使用规范核苷酸生成金属碱基对的可能性，然后描述了如何在这种情况下使用修饰核苷酸。我们还提供了用于分析金属碱基对的性质和形成的主要分析技术的描述，以及它们应用的相关实例。
