Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang Liaoning Province, People's Republic of China.
Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai Shangdong Province, People's Republic of China.
J Pharm Pharmacol. 2021 Mar 1;73(1):110-117. doi: 10.1093/jpp/rgaa015.
Inflammation widely exists in many diseases and poses a great threat to human and animal health. Rutin, quercetin-3-rhamnosyl glucoside, has a variety of pharmacological effects, including anti-oxidant, anti-inflammatory, antibacterial, anticancer and radioresistance effects. The current study focused on evaluation of its anti-inflammatory activity and described the mechanism of rutin in lipopolysaccharide-induced RAW 264.7 cells.
The related gene and protein expression levels were investigated by quantification real-time PCR and western blotting, respectively.
This study revealed that rutin can decrease inducible nitric oxide synthase (iNOS) gene and protein expression levels, effectively increase IκB gene expression, reduce toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor-associated factor 6 (TRAF6) and p65 gene expression and inhibit the phosphorylation of IκB and p65 and the proteins expression of TLR4, MyD88 and TRAF6.
These results suggest that rutin might exert anti-inflammatory effect on LPS-stimulated RAW 264.7 cells and will be potentially useful as an adjuvant treatment for inflammatory diseases.
炎症广泛存在于多种疾病中，对人类和动物的健康构成巨大威胁。芦丁，槲皮素-3-鼠李糖苷，具有多种药理作用，包括抗氧化、抗炎、抗菌、抗癌和抗辐射作用。本研究重点评估了芦丁的抗炎活性，并描述了芦丁在脂多糖诱导的 RAW 264.7 细胞中的作用机制。
通过定量实时 PCR 和 Western blot 分别研究相关基因和蛋白的表达水平。
本研究表明，芦丁可降低诱导型一氧化氮合酶（iNOS）基因和蛋白的表达水平，有效增加 IκB 基因的表达，降低 Toll 样受体 4（TLR4）、髓样分化因子 88（MyD88）、肿瘤坏死因子受体相关因子 6（TRAF6）和 p65 基因的表达，并抑制 IκB 和 p65 的磷酸化以及 TLR4、MyD88 和 TRAF6 的蛋白表达。
这些结果表明，芦丁可能对 LPS 刺激的 RAW 264.7 细胞发挥抗炎作用，并可能作为炎症性疾病的辅助治疗药物。
