TCM and Ethnomedicine Innovation & Development International Laboratory, Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, PR China.
The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, PR China.
J Pharm Pharmacol. 2021 Mar 1;73(1):60-69. doi: 10.1093/jpp/rgaa029.
To explore the in vivo anti-obesity effect of chikusetsusaponin V and explore the underlying mechanism by transcriptomic and metabonomic methods.
The physiological parameters of high-fat-diet induced obese mice administered with or without 25 mg/kg and 100 mg/kg of chikusetsusaponin V by gavage for 16 weeks were recorded. In addition, the RNA-sequencing and UHPLC-Q-TOF techniques were applied to obtain the transcriptomic and metabolomic profiling, respectively.
Chikusetsusaponin V could significantly alleviate the high-fat-diet induced increase in the weight of the whole body and obesity-related organs or tissues, and ameliorate the lipid content in the blood, the lipid accumulation in the livers, as well as the hypertrophy of the fat tissues. Importantly, transcriptomic results revealed that more than 30 genes involved in the pathway which closely associates with obesity, were significantly altered. Moreover, metabolomic data indicated the key differential metabolites enriched in the pathways such as the activated protein kinase signaling pathway which is a vital mediator of obesity and other processes.
The integrative analysis highlighted that chikusetsusaponin V significantly influenced the activated protein kinase signaling pathway at both transcriptomic and metabolomic levels, thereby exerting anti-obesity effects.
通过转录组学和代谢组学方法探索柴胡皂苷 V 的体内抗肥胖作用及其作用机制。
记录灌胃给予 25mg/kg 和 100mg/kg 柴胡皂苷 V 的高脂饮食诱导肥胖小鼠 16 周后的生理参数。此外，应用 RNA-seq 和 UHPLC-Q-TOF 技术分别获得转录组学和代谢组学图谱。
柴胡皂苷 V 可显著减轻高脂饮食引起的体重和肥胖相关器官或组织的增加，并改善血液中的脂质含量、肝脏中的脂质积累以及脂肪组织的肥大。重要的是，转录组学结果表明，超过 30 个与肥胖密切相关的通路基因发生了显著改变。此外，代谢组学数据表明，在激活蛋白激酶信号通路等途径中，关键差异代谢物被富集，该通路是肥胖和其他过程的重要介质。
综合分析强调，柴胡皂苷 V 在转录组学和代谢组学水平上显著影响激活蛋白激酶信号通路，从而发挥抗肥胖作用。
