Saint-Petersburg State Chemical Pharmaceutical University, Prof. Popov str. 14, Saint-Petersburg 197376, Russia.
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, Moscow 127276, Russia.
Nutrients. 2023 Jan 28;15(3):656. doi: 10.3390/nu15030656.
Obesity, and its consequences for human health, is a huge and complicated problem that has no simple solution. The constant search for natural and safe compounds with systemic action that can be used for obesity prophylactics and treatment is hampered by the limited availability and variable quality of biomass of wild medicinal plants. Plant cell biotechnology is an alternative approach for the sustainable production of vegetative biomass or individual phytochemicals with high therapeutic potential. In this study, the suspension cell biomass of the medicinal plants, Wall., L., and (T. Nees) C.A. Mey, produced in 20 L and 630 L bioreactors, were tested for therapeutic effects in rat models with alimentary-induced obesity. Three-month intake of water infusions of dry cell biomass (100 mg/g body weight) against the background of a hypercaloric diet reduced weight gain and the proportion of fat mass in the obese animals. In addition, cell biomass preparation reduced the intracellular dehydration and balanced the amounts of intra- and extracellular fluids in the body as determined by bioimpedance spectroscopy. A significant decrease in the glucose and cholesterol levels in the blood was also observed as a result of cell biomass administration for all species. Hypocholesterolemic activity reduced in the line > > /liraglutide > intact group > control group. By the sum of parameters tested, the cell culture of was considered the most effective in mitigating diet-induced obesity, with positive effects sometimes exceeding those of the reference drug liraglutide. A safety assessment of cell phytopreparation showed no toxic effect on the reproductive function of the animals and their offspring. These results support the potential application of the biotechnologically produced cell biomass of medicinal plant species as safe and effective natural remedies for the treatment of obesity and related complications, particularly for the long-term treatment and during pregnancy and lactation periods when conventional treatment is often contraindicated.
肥胖及其对人类健康的影响是一个巨大而复杂的问题，没有简单的解决方案。由于野生药用植物生物量的有限可用性和可变质量，人们不断寻找具有全身作用的天然和安全化合物，用于肥胖的预防和治疗，但受到阻碍。植物细胞生物技术是一种替代方法，可以可持续地生产具有高治疗潜力的植物生物量或单个植物化学物质。在这项研究中，在 20 L 和 630 L 生物反应器中生产的药用植物 Wall.、L. 和 (T. Nees) C.A. Mey 的悬浮细胞生物量，在具有饮食诱导肥胖的大鼠模型中进行了治疗效果测试。在高热量饮食的背景下，三个月摄入干细胞生物质的水浸液（100mg/g 体重）可减少肥胖动物的体重增加和脂肪量比例。此外，细胞生物质制剂通过生物阻抗谱法减少了细胞内脱水并平衡了体内的细胞内和细胞外液量。由于细胞生物质的给药，还观察到血液中葡萄糖和胆固醇水平的显著降低。对于所有物种，降胆固醇活性均降低 > > /liraglutide > 完整组 > 对照组。根据测试的参数总和，认为 的细胞培养在缓解饮食诱导的肥胖方面最有效，其积极作用有时超过参考药物利拉鲁肽。对 细胞植物制剂的安全性评估显示，对动物及其后代的生殖功能没有毒性作用。这些结果支持生物技术生产的药用植物物种的细胞生物量作为安全有效的天然药物治疗肥胖及其相关并发症的潜在应用，特别是在长期治疗以及妊娠和哺乳期，此时常规治疗通常是禁忌的。
