Effect of metabolic uncouplers on the performance of toluene-degrading biotrickling filter.

The biomass control potential of three metabolic uncouplers (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), carbonyl cyanide m-chlorophenylhydrazone (CCCP), and m-chlorophenol (m-CP)) was tested in biotrickling filters (BTFs) degrading toluene. The experiments employed two types of reactors: a traditional column design and a novel differential BTF (DBTF) reactor developed by De Vela and Gostomski (J Environ Eng 147:04020159, 2021). Uncouplers caused the toluene elimination capacity (EC) (~33 g/mh for column reactors and ~600 g/mh for DBTF) to decrease by 15-97% in a dose-dependent fashion. The EC completely recovered in the column reactor in 3 to 13 days, while only partial recovery happened in the DBTF. Short-term (1 to 3 days) true uncoupling was indicated by the 20-160% increase in %CO recovery, depending on concentration. FCCP and CCCP increased the pressure drop due to increased extracellular polymeric substances (EPS) production for protection against the uncouplers. The 4.0-mM m-CP weakened the biofilm in the BTF bed, as evidenced by the 130-500% increase in the total organic carbon in the liquid sump of the column and DBTF reactors. Moreover, a microbial shift led to the proliferation of genera that degrade uncouplers, further demonstrating that the uncouplers tested were not a sustainable biomass control strategy in BTFs.