Xu Xiangdong
School of Public Health and Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, China.
Adv Exp Med Biol. 2021;1280:189-200. doi: 10.1007/978-3-030-51652-9_13.
This chapter presents the fundamentals, instrumentation, methodology, and applications of capillary electrophoresis-mass spectrometry (CE-MS) for cancer metabolomics. CE offers fast and high-resolution separation of charged analytes from a very small amount of sample. When coupled to MS, it represents a powerful analytical technique enabling identification and quantification of metabolites in biological samples. Several issues need to be addressed when combining CE with MS, especially the interface between CE and MS and the selection of a proper separation methodology, sample pretreatment, and capillary coatings. We will discuss these aspects of CE-MS and detail representative applications for cancer metabolomic analysis.
本章介绍了用于癌症代谢组学的毛细管电泳-质谱联用（CE-MS）的基本原理、仪器设备、方法及应用。毛细管电泳能从极少量样品中对带电分析物进行快速、高分辨率的分离。与质谱联用时，它是一种强大的分析技术，可对生物样品中的代谢物进行鉴定和定量。将毛细管电泳与质谱联用时有几个问题需要解决，尤其是毛细管电泳与质谱之间的接口以及合适的分离方法、样品预处理和毛细管涂层的选择。我们将讨论毛细管电泳-质谱联用的这些方面，并详细介绍癌症代谢组学分析的代表性应用。
