Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
Adv Exp Med Biol. 2021;1336:159-178. doi: 10.1007/978-3-030-77252-9_9.
Capillary electrophoresis-mass spectrometry (CE-MS) is a very useful analytical technique for the selective and highly efficient profiling of polar and charged metabolites in a wide range of biological samples. Compared to other analytical techniques, the use of CE-MS in metabolomics is relatively low as the approach is still regarded as technically challenging and not reproducible. In this chapter, the possibilities of CE-MS for metabolomics are highlighted with special emphasis on the use of recently developed interfacing designs. The utility of CE-MS for targeted and untargeted metabolomics studies is demonstrated by discussing representative and recent examples in the biomedical and clinical fields. The potential of CE-MS for large-scale and quantitative metabolomics studies is also addressed. Finally, some general conclusions and perspectives are given on this strong analytical separation technique for probing the polar metabolome.
毛细管电泳-质谱联用（CE-MS）是一种非常有用的分析技术，可用于选择性地高效分析广泛生物样本中的极性和带电代谢物。与其他分析技术相比，CE-MS 在代谢组学中的应用相对较少，因为该方法仍被认为具有技术挑战性且不可重现。在本章中，我们将特别强调最近开发的接口设计，突出 CE-MS 在代谢组学中的可能性。通过讨论生物医学和临床领域的代表性和最新实例，展示了 CE-MS 用于靶向和非靶向代谢组学研究的实用性。还讨论了 CE-MS 用于大规模和定量代谢组学研究的潜力。最后，就该强大的分析分离技术在探测极性代谢组方面给出了一些普遍的结论和展望。
