Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.
Université de Lorraine, CNRS, INSERM, IBSLOR, F-54000 Nancy, France.
Methods Mol Biol. 2021;2300:11-16. doi: 10.1007/978-1-0716-1386-3_2.
Precipitation is a critical step to recover RNA of high purity. This chapter describes the principles of alcoholic precipitation as well as a standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.
沉淀是获得高纯度 RNA 的关键步骤。本章介绍了醇沉淀的原理以及标准的基本方案,并提供了一些关键的注意事项,但也讨论了许多变体。实际上,需要考虑几个重要参数,如盐、醇或载体的选择,以提高沉淀效率和 RNA 回收率。