College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, PR China.
College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing, 100193, PR China.
Angew Chem Int Ed Engl. 2021 Jun 1;60(23):13042-13049. doi: 10.1002/anie.202103458. Epub 2021 May 5.
We report the development of a highly sensitive ratiometric fluorescent lateral flow immunoassay (RFLFIA) strip for rapid and accurate detection of acute myocardial infarction biomarker, namely heart-type fatty acid binding protein (H-FABP). The RFLFIA strip works in terms of ratiometric change of fluorescence signal, arising from blending of fluorescence emitted by two composite nanostructures conjugated to capture and probe antibodies and inner filter effect of gold nanoparticles. In conjunction with using custom smartphone-based analytical device and tonality analysis, quantitative detection of H-FABP was achieved with a low limit of detection at 0.21 ng mL . The RFLFIA strip can generate a visually distinguishable green-to-red color change around the threshold concentration of H-FABP (6.2 ng mL ), thus allowing the semi-quantitative diagnosis by the naked eye.
我们开发了一种高灵敏度的比率荧光侧向流动免疫分析（RFLFIA）条带，用于快速准确地检测急性心肌梗死生物标志物，即心脏型脂肪酸结合蛋白（H-FABP）。该 RFLFIA 条带基于荧光信号的比率变化，这源于与捕获和探测抗体偶联的两种复合纳米结构发出的荧光和金纳米粒子的内滤效应的混合。结合使用定制的基于智能手机的分析设备和色调分析，可以以低至 0.21ngmL 的检测限定量检测 H-FABP。该 RFLFIA 条带在 H-FABP 的阈值浓度（6.2ngmL）周围产生明显的绿到红颜色变化，因此可以通过肉眼进行半定量诊断。
