Opt Lett. 2021 Apr 1;46(7):1538-1541. doi: 10.1364/OL.413030.
Single molecule fluorescence tracking provides information at nanometer-scale and millisecond-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address this aspect by developing a theoretical foundation for a spectroscopy of interaction times, i.e., the inference of interaction from imaging data. A non-trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted, together with the dependence of the exponential term upon the microscopic reaction affinity. Our approach is validated with simulated and experimental datasets.
单分子荧光跟踪技术可在纳米级和毫秒级时间分辨率下提供生物环境中单个分子的动力学和相互作用信息。虽然可以很好地描述孤立分子的动态行为，但当两个不可区分的分子发生相互作用时，定量分析的结果就会受到限制。我们通过为相互作用时间光谱学（即通过成像数据推断相互作用）建立理论基础来解决这一问题。我们强调了相互作用时间分布从幂律到指数行为的非平凡转变，以及指数项对微观反应亲和力的依赖性。我们的方法通过模拟和实验数据集得到了验证。
