瑞香狼毒地上部分的抗炎作用。

Anti-inflammatory effect of Adelia ricinella L. aerial parts.

机构信息

Pharmacy Department, Universidad de Oriente, Santiago de Cuba, Cuba.

Center of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Santiago de Cuba, Cuba.

出版信息

J Pharm Pharmacol. 2021 Mar 8;73(4):553-559. doi: 10.1093/jpp/rgaa057.

DOI:10.1093/jpp/rgaa057

PMID:33793832

Abstract

OBJECTIVE

To investigate the main chemical components and the anti-inflammatory activity of extracts of Adelia ricinella L. aerial parts.

METHODS

Three extracts obtained by soxhlet extraction and ethanol/water mixtures were evaluated in their chemical composition by UPLC-DAD-MS/MS. The in vitro anti-inflammatory activity of the prepared extracts was assessed through three different assays: COX-1 and COX-2 enzymatic inhibition, cell-based COX assays on RAW264.7 macrophages (ATCC) measuring the COX-2 protein expression by Western blot and the measurement of the PGE2 concentration in the supernatants of the culture medium. Also was determinate the effect of the three extracts on the RAW 264.7 cell viability.

KEY FINDINGS

Few differences in the phytochemical profile were found between the three prepared extracts, identifying a blend of thirteen flavonoids derived from luteolin and apigenin, with orientin as main constituent. Plant extracts (alcoholic and aqueous) did not affect the macrophage cell viability (IC50 > 256 μg/ml) and significantly reduced COX-1 and COX-2 enzyme activities. Additionally, COX-2 expression and PGE2 release were suppressed after 24 h of LPS stimulation and treatment with plant extracts (8-64 µg/ml).

CONCLUSIONS

A. ricinella extracts showed the ability to reduce the inflammatory effect exerted by LPS in murine macrophages. However, further studies should confirm their anti-inflammatory activity.

摘要

目的

研究 Adelia ricinella L. 地上部分提取物的主要化学成分和抗炎活性。

方法

通过索氏提取和乙醇/水混合物获得的三种提取物，通过 UPLC-DAD-MS/MS 评估其化学成分。通过三种不同的测定方法评估制备提取物的体外抗炎活性：COX-1 和 COX-2 酶抑制、基于细胞的 RAW264.7 巨噬细胞（ATCC）测定，通过 Western blot 测定 COX-2 蛋白表达，以及测量培养基上清液中 PGE2 的浓度。还测定了三种提取物对 RAW 264.7 细胞活力的影响。

主要发现

三种制备提取物之间的植物化学成分谱差异不大，鉴定出 13 种源自木犀草素和芹菜素的类黄酮混合物，以橙皮苷为主要成分。植物提取物（酒精和水）不影响巨噬细胞活力（IC50 > 256 μg/ml），并显著降低 COX-1 和 COX-2 酶活性。此外，在用 LPS 刺激和用植物提取物（8-64 µg/ml）处理 24 小时后，COX-2 表达和 PGE2 释放受到抑制。