Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas, Campinas, SP, Brazil.
CNRS- Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France.
J Pharm Pharmacol. 2021 Mar 6;73(3):377-387. doi: 10.1093/jpp/rgaa030.
To evaluate the antibacterial, antifungal and anthelmintic activities of the ethanolic extract (EEMz), fractions (LPFMz and HPFMz) and compounds isolated from the leaves of Manilkara zapota L. P. Royen.
Extract and fractions were produced by turbolization. LPFMz fraction was analysed by gas chromatography-mass spectrometry. The isolated compounds from HPFMz were purified by flash and preparative chromatographic methods, and chemically characterised by UPLC-ESITOFMS, optical rotation, and one- and two-dimensional 1H and 13C NMR techniques. Anthelmintic against Strongyloides venezuelensis and antimicrobial activities against Candida albicans, Trichophyton rubrum and Staphylococcus aureus were evaluated.
EEMz showed mainly phenolic compounds and pentacyclic triterpenes from Δ12-oleane/Δ12-ursane series. Chlorogenic acid, myricetin-3-O-β-D-glucopyranoside, mearnsitrin, germanicol and germanicol acetate were reported to M. zapota leaves for the first time in this work. EEMz, HPFMz, LPFMz showed significative activity against C. albicans (16 µg/mL), while isolated flavonoids were active against S. aureus (<32 µg/mL). EEMz, phenolic-rich compounds (F2), and chlorogenic acid were potentially active against S. venezuelensis at 24 h.
M. zapota and its bioactive compound can be eligible such as a potential phytomedicine for the treatment of microbial and strongyloidiasis drug-resistant infections.
评估来自人心果树（Manilkara zapota L. P. Royen）叶的乙醇提取物（EEMz）、馏分（LPFMz 和 HPFMz）和分离化合物的抗菌、抗真菌和驱虫活性。
通过涡旋法生产提取物和馏分。对 LPFMz 馏分进行气相色谱-质谱分析。从 HPFMz 中分离出的化合物通过快速和制备色谱方法进行纯化，并通过超高效液相色谱-电喷雾离子阱飞行时间质谱、旋光度和一维和二维 1H 和 13C NMR 技术进行化学表征。评估了对委内瑞拉旋毛虫的驱虫活性和对白色念珠菌、红色毛癣菌和金黄色葡萄球菌的抗菌活性。
EEMz 主要显示出酚类化合物和五环三萜类化合物，来自Δ12-齐墩烷/Δ12-乌苏烷系列。在这项工作中，首次报道了人心果树叶中的绿原酸、杨梅素-3-O-β-D-吡喃葡萄糖苷、美那斯他汀、日耳曼醇和日耳曼醇乙酸酯。EEMz、HPFMz、LPFMz 对白色念珠菌（16 µg/mL）表现出显著的活性，而分离出的类黄酮对金黄色葡萄球菌（<32 µg/mL）具有活性。EEMz、富含酚类化合物（F2）和绿原酸在 24 小时内对委内瑞拉旋毛虫具有潜在活性。
人心果及其生物活性化合物可作为治疗微生物和旋毛虫病耐药感染的潜在植物药。
