利用高通量测序技术测定多棘海盘车（赫布斯特，1785年）的完整线粒体基因组
The complete mitochondrial genome of spiny spooner (Herbst, 1785) using high-throughput sequencing.
作者信息
Liu Hongtao, Shen MingHui
机构信息
Ministry of Education, Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources (Hainan Tropical Ocean University), Sanya, China.
Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.
出版信息
Mitochondrial DNA B Resour. 2021 Mar 18;6(3):985-987. doi: 10.1080/23802359.2021.1891982.
The whole mitochondrial genome of the spiny spooner collected from the South China Sea was determined for the first time using high-throughput sequencing. The circular mitogenome of is 15,884 bp, with 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes. The base composition is significantly biased (A, G, T, and C was 37.9%, 17.8%, 34.0%, and 10.3%, respectively) with A + T contents of 71.9%. Among 13 PCGs, all PCGs use a normal ATN as the start codon, and ten of them end with TAA or TAG except COX1, COX2 and CYTB gene using a single T as the stop codon. The phylogenetic tree showed that forms a cluster while form a sister group to this cluster.
首次利用高通量测序技术测定了从南海采集的刺勺形藻的完整线粒体基因组。其环状线粒体基因组为15,884 bp，包含13个蛋白质编码基因（PCGs）、22个tRNA基因和2个rRNA基因。碱基组成存在显著偏差（A、G、T和C分别为37.9%、17.8%、34.0%和10.3%），A+T含量为71.9%。在13个PCGs中，所有PCGs均以正常的ATN作为起始密码子，其中10个以TAA或TAG结尾，除COX1、COX2和CYTB基因以单个T作为终止密码子。系统发育树显示，[具体物种名称1]形成一个聚类，而[具体物种名称2]形成该聚类的姐妹群。
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