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多棘红鱼(耶格,1833年)线粒体全基因组的特征分析及系统发育分析

Characterization and phylogenetic analysis of the complete mitochondrial genome of prickly redfish (Jaeger, 1833).

作者信息

Liu Hongtao, Shen Minghui

机构信息

Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, China.

出版信息

Mitochondrial DNA B Resour. 2021 Jul 6;6(8):2275-2277. doi: 10.1080/23802359.2021.1920509. eCollection 2021.

DOI:10.1080/23802359.2021.1920509
PMID:34286097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8266223/
Abstract

In this study, the complete mitochondrial genome (mitogenome) of prickly redfish (Jaeger, 1833) was determined and characterized from the South China Sea using next-generation sequencing. Our results showed that the length of the whole mitogenome in prickly redfish was 15,858 bp and the mitogenome was consist of 22 tRNA genes, two rRNA genes, 13 protein-coding genes (PCGs), and one control region. Furthermore, the nucleotide composition was significantly biased (composition of A, G, T, and C was 28.20%, 22.64%, 33.53%, and 15.63%, respectively) with AT contents of 61.73%. All the PCGs shared a standard initiation codon ATG or GTG. and genes terminated with an incomplete stop codon T, while others ended with TAA or TAG. Our phylogenetic analysis showed that was clustered with the species of genus and formed sister branches with species of other genera within the family Stichopodidae clade.

摘要

在本研究中,利用二代测序技术测定并分析了采自中国南海的棘红鱼(Jaeger,1833)的完整线粒体基因组(线粒体基因组)。我们的结果表明,棘红鱼的线粒体基因组全长15,858 bp,由22个tRNA基因、2个rRNA基因、13个蛋白质编码基因(PCGs)和1个控制区组成。此外,核苷酸组成存在显著偏差(A、G、T和C的组成分别为28.20%、22.64%、33.53%和15.63%),AT含量为61.73%。所有的PCGs都共享标准起始密码子ATG或GTG, 基因以不完全终止密码子T结束,而其他基因以TAA或TAG结束。我们的系统发育分析表明, 与 属的物种聚类,并与刺参科分支内其他属的物种形成姐妹分支。 (原文中存在部分未明确表述的内容,已尽量按照正确逻辑翻译)

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4e/8266223/2197471b0d7c/TMDN_A_1920509_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4e/8266223/2197471b0d7c/TMDN_A_1920509_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4e/8266223/2197471b0d7c/TMDN_A_1920509_F0001_B.jpg

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本文引用的文献

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Mitochondrial DNA B Resour. 2019 Oct 1;4(2):3305-3306. doi: 10.1080/23802359.2019.1673244.
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Isolation and characterization of 42 microsatellite loci from the prickly redfish Thelenota ananas (Echinodermata, Stichopodidae).从刺鲀(棘皮动物,鳞鲀科)中分离和特征分析 42 个微卫星位点。
Mol Biol Rep. 2019 Oct;46(5):5569-5574. doi: 10.1007/s11033-019-04957-7. Epub 2019 Jul 4.
3
IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.
IQ-TREE:一种用于估计最大似然系统发育树的快速且有效的随机算法。
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Molecular taxonomy, phylogeny and evolution in the family Stichopodidae (Aspidochirotida: Holothuroidea) based on COI and 16S mitochondrial DNA.基于 COI 和 16S 线粒体 DNA 的锚参科(Aspidochirotida:海参纲)分子分类学、系统发育和进化。
Mol Phylogenet Evol. 2010 Sep;56(3):1068-81. doi: 10.1016/j.ympev.2010.04.013. Epub 2010 Apr 23.