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(蔷薇科)变种的叶绿体基因组特征及系统发育定位

Chloroplast genome features and phylogenomic placement of var. (Rosaceae).

作者信息

Liu Dan, Xie Xiao-Man, Lu Yi-Zeng, Li Feng, Zheng Jian, Yang Hai-Ping, Liu Kun, Zhang Xiao-Bo, Li Wen-Qing

机构信息

College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, People's Republic of China.

Shandong Forest Germplasm Resources Center, Ji'nan, Shandong Province, People's Republic of China.

出版信息

Mitochondrial DNA B Resour. 2021 Mar 18;6(3):1068-1070. doi: 10.1080/23802359.2021.1899859.

DOI:10.1080/23802359.2021.1899859
PMID:33796741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7995867/
Abstract

The complete chloroplast genome of var. was sequenced with Illumina HiSeq 2000 platform. It was a typical quadruple structure as other plants of with 160,050 bp in length, including a large single-copy (LSC: 87,905 bp) region and a small single-copy (SSC: 19,325 bp) which were separated by a pair of inverted repeats (IRa, b: 26,410 bp) region. The overall GC content is 36.5%. A total of 130 genes was annotated which contained 85 protein-coding genes including the Trans splicing gene of 12, 37 tRNA genes, and 8 rRNA genes. ML phylogenetic analysis compared with 7 expressed chloroplast genomes of Rosaceae revealed that var. was a sister to other species. Six species of were divided into two groups, the species of group one is distributed in Asia and the species of group two distributed in Europe. Among group one, var. had the closest genetic relationship with which is a New Endemic Species on Ulleung Island of Korea, and followed by which is only distributed in Sichuan and Guizhou of China. var. has a relatively close relationship with the other three species of in the group two. And, it has a relatively distant from other genera of and .

摘要

采用Illumina HiSeq 2000平台对[变种名称]的完整叶绿体基因组进行了测序。它具有与[属名]其他植物一样的典型四分体结构,长度为160,050 bp,包括一个大单拷贝(LSC:87,905 bp)区域和一个小单拷贝(SSC:19,325 bp)区域,这两个区域被一对反向重复序列(IRa、b:26,410 bp)区域隔开。总体GC含量为36.5%。共注释了130个基因,其中包括85个蛋白质编码基因(含12个反式剪接基因)、37个tRNA基因和8个rRNA基因。与蔷薇科7个已发表的叶绿体基因组进行的最大似然法系统发育分析表明,[变种名称]是其他[属名]物种的姐妹种。[属名]的六个物种分为两组,第一组的物种分布在亚洲,第二组的物种分布在欧洲。在第一组中,[变种名称]与韩国郁陵岛的新特有物种[物种名称]遗传关系最近,其次是仅分布在中国四川和贵州的[物种名称]。[变种名称]与第二组中的其他三个[属名]物种关系相对较近。并且,它与[属名]和[属名]的其他属亲缘关系相对较远。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db42/7995867/30937ab5bd26/TMDN_A_1899859_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db42/7995867/30937ab5bd26/TMDN_A_1899859_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db42/7995867/30937ab5bd26/TMDN_A_1899859_F0001_B.jpg

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Chloroplast genome features and phylogenomic placement of var. (Rosaceae).(蔷薇科)变种的叶绿体基因组特征及系统发育定位
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本文引用的文献

1
IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.IQ-TREE:一种用于估计最大似然系统发育树的快速且有效的随机算法。
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Nucleic Acids Res. 2013 Jul;41(13):e129. doi: 10.1093/nar/gkt371. Epub 2013 May 9.
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MAFFT 多序列比对软件版本 7:性能和易用性的改进。
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