Carter H B, Coffey D S
Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
J Urol. 1988 Jul;140(1):173-5. doi: 10.1016/s0022-5347(17)41521-7.
The transplantable Dunning R-3327 rat prostatic adenocarcinoma model has provided a series of tumor variants with broad ranges of metastatic potential. We tested whether cell surface charge might be related to metastatic potential by measuring the electrophoretic mobility of live tumor cells obtained by needle aspiration. Cells were aspirated from tumors with low metastatic potential (following subcutaneous inoculation of 10(6) tumor cells the H, G and AT-1 variants had less than 5% metastases; AT-2 had 5-20%) and were compared to the electrophoretic mobility of cells aspirated from highly metastatic tumors (MAT-LyLu, MAT-Lu, AT-3 had greater than 90% metastases). Electrophoretic mobility expressed in mu/sec/volt/cm. was measured on 100 cells from each tumor subline, and the cell surface charge expressed as a zeta potential was calculated from electrophoretic mobility using the Helmholtz-Smoluchowski equation. The average zeta potential (+/- S.E.M.) for the four sublines with low metastatic potential was (-17.4 +/- 0.4 mV) compared to the three sublines with high metastatic potential (-26.5 +/- 0.7 mV), and the differences were significant (p less than .01) using the Mann-Whitney Wilcoxon test. Using a zeta potential of -20.5 mV as the cutoff between high and low metastatic potential, the sensitivity and specificity of zeta potential in predicting metastatic potential in 140 determinations on seven tumor lines were 92% and 82.5%, respectively. The predictive value of a positive test (value greater than -20.5 mV) was 80% and the predictive value of a negative test (value less than -20.5 mV) was 93%. The results support a difference in the cell surface charge between these metastatic and nonmetastatic tumors with increasing negativity at the cell surface correlating with increased metastatic potential, but not with tumor growth rates.
可移植的Dunning R - 3327大鼠前列腺腺癌模型提供了一系列具有广泛转移潜能的肿瘤变体。我们通过测量针吸获得的活肿瘤细胞的电泳迁移率，来测试细胞表面电荷是否可能与转移潜能相关。从低转移潜能的肿瘤中吸取细胞（皮下接种10⁶个肿瘤细胞后，H、G和AT - 1变体的转移率低于5%；AT - 2为5 - 20%），并与从高转移潜能肿瘤中吸取的细胞的电泳迁移率进行比较（MAT - LyLu、MAT - Lu、AT - 3的转移率大于90%）。以微米/秒/伏/厘米表示的电泳迁移率在每个肿瘤亚系的100个细胞上进行测量，并使用亥姆霍兹 - 斯莫卢霍夫斯基方程根据电泳迁移率计算表示为ζ电位的细胞表面电荷。四个低转移潜能亚系的平均ζ电位（±标准误）为（-17.4 ± 0.4毫伏），与之相比，三个高转移潜能亚系为（-26.5 ± 0.7毫伏），使用曼 - 惠特尼 - 威尔科克森检验，差异具有统计学意义（p < 0.01）。以-20.5毫伏的ζ电位作为高转移潜能和低转移潜能的分界点，在对七个肿瘤系的140次测定中，ζ电位预测转移潜能的敏感性和特异性分别为92%和82.5%。阳性检测（值大于-20.5毫伏）的预测值为80%，阴性检测（值小于-20.5毫伏）的预测值为93%。结果支持这些转移性和非转移性肿瘤之间细胞表面电荷存在差异，细胞表面负电荷增加与转移潜能增加相关，但与肿瘤生长速率无关。
